The first subacute study with oral administration of bisphenol AP in male rats : cardiovascular data and metabolite detection

Mladěnka, Přemysl (author), Fadraersada, Jaka (author), Dias, Patrícia (author), Tvrdý, Václav (author), Pourová, Jana (author), Jirkovský, Eduard (author), Carazo, Alejandro (author), Sollner Dolenc, Marija (author), Trontelj, Jurij (author), Peterlin-Mašič, Lucija (author)

Bisphenols are frequently used in the plastic industry. However, there is a need for addressing their safety in in vivo studies based on the observed negative impact of bisphenol A. Bisphenol AP (BPAP) is frequently found in the environment, human food, and biological fluids, but its cardiovascular effects have not yet been tested. For this reason, we administered BPAP in a daily single dose of 2.5 mg.kg$^{-12}$ via oral gavage to eight male Wistar Han rats for a period of 4 weeks. Eight control rats received the solvent, sesame oil. Parent BPAP was detectable in plasma only in 3 animals by a validated LC-MS/MS method, whereas its glucuronide was found in all animals (48.4 ± 25.4 nM). Arterial blood pressure did not change during or at the end of the treatment. Some disturbances in cardiac rhythm were observed (e.g., increased heart rate, prolonged P wave and QT$_{peak}$ interval, p < 0.05), but there was no increase in serum cardiac troponin T. Vessels isolated from BPAP-treated animals showed impaired vasodilatory response compared to control animals (p < 0.05). BPAP impacted coagulation and caused hemolysis only in hundreds of µM concentrations. These results demonstrate the potential of BPAP to affect cardiac rhythm and impair vessel homeostasis after subacute exposure.

• Language: English
• Keywords: bisphenol, endocrine disruptor, pollutant, hypertension, cardiovascular
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: 9 str.
• Numbering: Vol. 304, art. 119143
• PID: 20.500.12556/RUL-175900
• UDC: 612.43:620.266.1
• ISSN on article: 0147-6513
• DOI: 10.1016/j.ecoenv.2025.119143
• COBISS.SI-ID: 251567875
• Publication date in RUL: 13.11.2025

Record is a part of a journal

• Title: Ecotoxicology and environmental safety
• Shortened title: Ecotoxicol. environ. saf.
• Publisher: Academic Press
• ISSN: 0147-6513
• COBISS.SI-ID: 25368320

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: bisfenol, endokrini motilci, onesnaževalci, hipertenzija, kardiovaskularni sistem, hormonski motilci