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Sodium chloride, colchicine, and 6-benzylaminopurine can change antioxidant property and phenols content in Hypericum perforatum L. : an in vitro study
ID Manteghi Tafreshi, Ara (Author), ID Mohammadhassan, Reza (Author)

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Abstract
Hypericum perforatum, a medicinal plant from the Hypericaceae family, is known for its wide range of bioactive properties, including antidepressant, antiviral, antibacterial, and anti-cancer activity. These effects are attributed mainly to its antioxidants and among them phenolic secondary metabolites. This in vitro study aimed to investigate the impact of various concentrations of colchicine (0.05, 0.1, 0.2 mg l-1), sodium chloride (NaCl; 0.5, 1. 2 mg l-1), and 6-benzylaminopu-rine (BAP; 0.25, 0.5, 1 mg l-1) on antioxidant activity and total phenol content in H. perforatum over three weeks. A factorial experiment was conducted in a completely randomized design with three replications. The results revealed that all treatments significantly enhanced (p < 0.01) antioxidant capacity, phenol content, and morphological characteristics. Notably, the highest levels of antioxidant activity and total phenol content were observed in the third week following treatment with 0.25 mg l-1 BAP, 2 mg l-1 NaCl, and 2 mg l-1 colchicine, separately. These findings suggest that the higher concentrations of NaCl and colchicine, along with a lower concentration of BAP, can effectively enhance the biosynthesis of phenolic compounds and antioxidant activity in H. perforatum.

Language:English
Keywords:Hypericum perforatum, antioxidant property, phenol content, colchicine, sodium chloride, 6-benzylaminopurine, plant tissue culture
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2025
Number of pages:12 str.
Numbering:Vol. 121, no. 3
PID:20.500.12556/RUL-175012 This link opens in a new window
UDC:63
ISSN on article:1854-1941
DOI:10.14720/aas.2025.121.3.18392 This link opens in a new window
COBISS.SI-ID:252950787 This link opens in a new window
Publication date in RUL:13.10.2025
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Downloads:236
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Record is a part of a journal

Title:Acta agriculturae Slovenica
Publisher:Biotehniška fakulteta, Oddelek za agronomijo in Oddelek za zootehniko, Biotehniška fakulteta, Oddelek za agronomijo in Oddelek za zootehniko, Univerza v Ljubljani, Biotehniška fakulteta, Založba Univerze v Ljubljani
ISSN:1854-1941
COBISS.SI-ID:219581184 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Title:Natrijev klorid, kolhicin in 6-benzilaminopurin lahko spremenijo antioksidacijske lastnosti in vsebnost fenolov v šen-tjanževki (Hypericum perforatum L.): in vitro raziskava
Abstract:
Šentjanževka (Hypericum perforatum L.), zdravilna rastlina iz družine krčničevk (Hypericaceae), je zelo poznana zaradi svojih bioaktivnih lastnosti kot so antidepresivna, protivirusna, protibakterijska in protirakovna aktivnost. Ti učinki so v glavnem posledica vsebnosti antioksidantov in med njimi fenolnih sekundarnih metabolitov. Namen te in vitro raziskave je bil preučiti vpliv različnih koncentracij kolhicina (0,05; 0,1; 0,2 mg l-1), natrijevega klorida (NaCl; 0,5; 1, 2 mg l-1) in 6-benzilaminopurina (BAP; 0,25; 0,5; 1 mg l-1) na antioksidacijsko aktivnost in vsebnost celokupnih fenolov v šentjanževki v obdobju treh tednov. Popolni naključni faktorski poskus je bil zasnovan v treh ponovitvah. Rezultati so pokazali, da so vsa obravnavanja značilno povečala (p < 0.01) antioksidacijsko sposobnost, vsebnost phenolov in morfološke lastnosti. Največji antioksidacijska aktivnost in vsebnost celokupnih fenolov sta bili ugotovljeni tretji teden po obravnavanjih z 0,25 mg l-1BAP, 2 mg l-1 NaCl in ločeno po obravnavanju z 2 mg l-1 kolhicina. Te ugotovitve nakazujejo, da lahko večje koncentracije NaCl in kolhicina hkrati z manjšimi koncentracijami BAP učinkovito pospešijo biosintezo fenolnih spojin in povečajo antioksidativno aktivnost šentjanževke.

Keywords:Hypericum perforatum, antioksidacijske lastnosti, vsebnost fenolov, kolhicin, natrijev klorid, 6-benzilaminopurin, rastlinske tkivne kulture

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