In this thesis, the influence of three agronomic practises on the morphological, agronomic, and biochemical properties of the first Slovenian hemp variety (Cannabis sativa L.) ‘Fiona’, officially registered in the national variety list in 2021, was tested. The main goal was to find out which methods have the greatest impact on seed and stem yield. The field trial was carried out in 2024, from the end of April to the end of September, near Krško, on a 2-hectare field. The seeds were sown at two sowing dates (end of April and end of May) at a rate of 30 kg/ha. Before sowing, the field was fertilised with 500 kg/ha of NPK fertiliser (6-12-14). In the treatment investigating the effect of additional nitrogen fertilisation, an additional 50 kg/ha of nitrogen (185 kg/ha NPK fertiliser) was applied at the three-pair leaf stage. In addition, a topping treatment was applied for plants sown on the first date at a plant height of 1.0 m and 1.2 m. Plants from the second sowing date were topped at a height of 1 m. At harvest, total plant height, stem diameter, height to the first branching point of topped plants, inflorescence length, and fresh stem biomass were measured. Stems and flowers were dried and then separated into seeds and dry biomass. The dry mass of the seeds and stems was determined, while the cannabinoid profile of the biomass (CBD and THC) was analysed by gas chromatography. The seed samples were also analysed for protein and oil content. The results show that sowing date was a decisive factor for seed yield, with later sowing leading to a 340 kg/ha higher yield compared to earlier sowing. Nitrogen fertilisation significantly increased yields, with fertilised plants producing almost 2 t/ha more stems and 217 kg/ha more seeds than unfertilised plants. Topping had a strong effect on plant morphology, as untopped plants were up to 1 m taller than topped plants. In contrast, biochemical responses were less variable compared to morphological traits. The nutritional quality of the seeds was high, with an average content of 23.43% protein and 31.94% oil. The CBD content in the post-harvest biomass was around 1.3%.
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