In this experiment,, we studied the effect of biostimulants on the quantity and quality of lettuce (Lactuca sativa L.) under different water supply conditions. The experiment was conducted in 2024 at the Rozman farm in Križe nar Tržič on three consecutive dates: May 13, 2024 - June 26, 2024, June 21, 2024 - August 6, 2024, and August 5, 2024 - September 16, 2024. In the study, we used the 'Champollion' lettuce variety, the Delfan plus biostimulant, which contains amino acids, and the Humistar biostimulant, which contains humic acids. In the first period, we carried out four treatments, in the second period six treatments, and in the third period eight treatments, which included biostimulants and different irrigation regimes. We harvested the lettuce when it reached a market weight of 350 g and then weighed the fresh weight of the samples, the weight of the cleaned rosettes, measured the SPAD value, and determined the dry matter content. The results showed that the combination of humic acids and amino acids increased the yield in irrigated plants compared to treatment with amino acids alone, while in non-irrigated plants the yield with the addition of both biostimulants was simiilar to that with amino acids alone. The independent use of humic acids in the third term had no effect on yield. The effectiveness of biostimulants varied between growing terms, confirming the influence of environmental conditions. The best results were achieved in the second term with balanced temperatures and sufficient moisture. Amino acids proved to be more effective in non-irrigated plants, while the effect of humic acids could not be clearly confirmed.
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