Fluorescent molecules have played a key role in bioanalytics since the 1950s. In recent years, new approaches for functionalizing fluorescent molecular probes with nanoparticles have been developed, expanding the scope of potential applications. To functionalize fluorescent molecules, we first need to extend them with an appropriate linker before we can attach them to nanoparticles. One approach involves conjugating the elongated fluorescent molecule to biotin, which then allows it to bind to streptavidin-modified magnetic nanoparticles. In this bachelor’s thesis we successfully synthesised a fluorescent molecule and conjugated it to biotin via an ethylene glycol linker. The starting Boc-protected alcohol was elongated into a carboxylic acid through an esterification reaction. Subsequently, a fluorescent probe, obtained by a Henry reaction, was coupled to the carboxylic acid. Afterwards, the Boc protective group was removed and the released free amine linked to biotin, which had been activated with PyBOP prior to the reaction. The final products were purified and characterized.
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