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In vitro razgradnja hitozanskih filmov s komercialnimi encimi
ID Belak, Anej (Author), ID Likozar, Blaž (Mentor) More about this mentor... This link opens in a new window

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Abstract
Problem odpadne plastike je eden izmed večjih okoljskih problemov. Proizvodnja plastike le še narašča. Eden izmed možnih rešitev je razvoj novih materialov, sposobnih spontane razgradnje v okolju. Primer takšnega materiala je hitozan, pridobljen iz hitina, ki je osnovni gradnik ogrodja rakov in školjk. Hitozan je skupno ime za skupino hitinov, deacetiliranih do različnih stopenj. Diploma je bila napisana v sklopu projekta Microbeast. Raziskan je bil vpliv komercialnih encimov lakaze, celulaze, lipaze in alkohol dehidrogenaze na razgradnjo pripravljenih hitozanskih filmov v časovnem obdobju 48 h. Dani encimi so bili izbrani zaradi njihove dostopnosti in prisotnosti v prsti. Na podlagi rezultatov imata največji vpliv encima celulaza in lipaza, medtem ko alkohol dehidrogenaza in lakaza nista imela bistvenega vpliva. Hitozan je bil lažje razgradljiv pri pH 6 v primerjavi s pH 7.

Language:Slovenian
Keywords:encimi, biorazgradnja, hitozan, biopolimer
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Year:2025
PID:20.500.12556/RUL-172519 This link opens in a new window
COBISS.SI-ID:255174659 This link opens in a new window
Publication date in RUL:08.09.2025
Views:118
Downloads:21
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Secondary language

Language:English
Title:In vitro degradation of chitosan films by commercial enzymes
Abstract:
The problem of plastic waste is one of the biggest environmental problems. Plastic production continues to grow. One possible solution is the development of new materials capable of spontaneous degradation in the environment. An example of such a material is chitosan, obtained from chitin, which is the basic building block of the exoskeleton of crabs and shellfish. Chitosan is the collective name for a group of chitins deacetylated to varying degrees. The thesis was written as part of the Microbeast project. The influence of commercial enzymes Laccase, Cellulase, Lipase, and alcohol dehydrogenase on the degradation of prepared chitosan films over a period of 48 hours was investigated. These enzymes were selected because of their availability and presence in soil. Based on the results, cellulase and lipase had the greatest impact, while alcohol dehydrogenase and laccase had no significant effect. Chitosan was more easily degradable at pH 6 compared to pH 7.

Keywords:enzymes, biodegradation chitosan, biopolymer

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