Skeletal muscles are a complex organs that represent approximately 40 % of the human body mass. They enable our movement and posture, respond quickly to external stimuli, and store various nutrients. For proper function they require chemical energy. In mammals, skeletal muscles represent the main site for glucose deposition and glycogen storage for their needs. Glucose uptake in skeletal muscles is facilitated through transporters GLUT1 and GLUT4. When the glucose concentration is high, glycogen synthase 1 converts it into glycogen. During contraction muscle glycogen phosphorylase cleaves glycogen into glucose molecules. The environment is important for the adhesion, proliferation, differentiation, and migration of skeletal muscle cells, which is why efforts are made to provide similar conditions for our in vitro models. Our attempt was to provide the key components for the function of cultured primary human skeletal muscle cells. Examples of commercial extracellular matrixes that we used are Matrigel and Geltrex. The aim of our research was to examine the influence different well coatings on the expression of two enzymes, glycogen synthase 1 and muscle glycogen phosphorylase, and two transporters, GLUT1 and GLUT4. We hypothesized that Matrigel and Geltrex would increase the expression of glycogen synthase 1 (GYS1) and muscle glycogen phosphorylase (PYGM) and the transporters GLUT1 and GLUT4 in primary human skeletal muscle cells. The results partially confirmed our hypothesis. We discovered that there was an increased expression of the enzymes glycogen synthase 1 and muscle glycogen phosphorylase in cells plated on Matrigel or Geltrex matrix, but the amount of mRNA did not differ. The matrix did not impact the expression of transporters GLUT1 and GLUT4.
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