Confirmation of top cross hybrids in guava using morpho-molecular markers

Akhi, Masuma Zahan (author), Hassan, Jahidul (author), Raihan, Mohammad Sharif (author), Rahman, Md Mizanur (author), Biswas, Md. Sanaullah (author)

The study was conducted to confirm the genetic diversity and hybridity of seventeen guava progenies developed from top-crossing between genetically distinct green and purple guava varieties. Morphological, biochemical, and molecular markers effectively identified hybrids exhibiting phenotypes from both parents. Moreover, remarkable genetic diversity was revealed among these segregants. Biplot analysis demonstrated a strong positive relationship between: (1) chlorophyll and anthocyanin content, (2) leaf length-to-width ratio, (3) leaf area, and (4) petiole length, identifying G15 and G16 genotypes as superior top-cross hybrids. A set of 10 simple sequence repeat (SSR) markers identified 36 alleles with a mean of 3.6 alleles per primer. The polymorphism percentage was 80.83 %, with pairwise dissimilarity ranging from 0.071 to 0.357. Four SSR primers (mPgCIR03, mPgCIR08, mPgCIR11, and mPgCIR19) specifically confirmed the top-cross hybrid status of G6, G8, G9, G10, G15, and G16 genotypes. These diverse genetic resources will be maintained for homozygous plant production through selfing and subsequent guava improvement programs.

• Language: English
• Keywords: genetic diversity, top cross, polymorphism, dissimilarity index, molecular marker, segregates
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: 14 str.
• Numbering: Vol. 121, no. 2
• PID: 20.500.12556/RUL-170792
• UDC: 631
• ISSN on article: 1854-1941
• DOI: 10.14720/aas.2025.121.2.18689
• COBISS.SI-ID: 242686979
• Publication date in RUL: 16.07.2025

Record is a part of a journal

• Title: Acta agriculturae Slovenica
• Publisher: Biotehniška fakulteta, Oddelek za agronomijo in Oddelek za zootehniko, Biotehniška fakulteta, Oddelek za agronomijo in Oddelek za zootehniko, Univerza v Ljubljani, Biotehniška fakulteta, Založba Univerze v Ljubljani
• ISSN: 1854-1941
• COBISS.SI-ID: 219581184

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Title: Določitev najboljših križancev gvajave z morfološkimi in molekularnimi markerji

Abstract

Namen raziskave je bil potrditi genetsko raznolikost in hibridnost sedemnajstih potomcev gvajave pridoblejnih s križanjem genetsko različnih zelenih in škrlatnih sort. Morfološki, biokemični in molekularni markerji so potrdili, da izražajo križanci fenotipe obeh staršev, pri čemer je bila med njimi ugotovljena opazna genetska raznolikost. Biplotna analiza je pokazala močne pozitivne povezave med lastnostmi kot so: (1) vsebnost klorofila in antocianov, (2) razmerje med dolžino in širino listov, (3) v listni površini in (4) dolžini listnih pecljev. Pri tem sta bila genotipa G15 in G16 prepoznana kot najboljša križanca. Z naborom 10 markerjev enostavnih ponavljajočih se zaporedij (SSR) je bilo določenih 36 alelov, s poprečjem 3,6 alela na marker. Odstotek polimorfizma je bil 80,83 %, parna različnost je bila med 0,071 in 0,357. Štirje SSR primerji (mPgCIR03, mPgCIR08, mPgCIR11 in mPgCIR19) so še posebej potrdili najboljše križance med genotipi kot so G6, G8, G9, G10, G15 in G16. Ta raznolik genet-skih vir bo vzdrževan za vzgojo homozigotnih rastlin preko samoopraševanja v bodočih programih žlatnenja gvave.

• Keywords: genetska raznolikost, vrhunski križanci, polimorfizem, indeks različnosti, molekularni marker, segregacija

Projects

• Funder: BSMRAU - Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh