Standard operating procedure for interferometric light microscopy of extracellular particles within the nanostructurome pipeline

Romolo, Anna (author), Kralj-Iglič, Veronika (author)

A standard operating procedure (SOP) for assessment of number density and hydrodynamic diameter of particles sized between cca 80 nm and 500 nm is presented. The aim of this SOP is to provide characterization of extracellular particles (EPs) which enables analysis of large cohorts of minimally processed samples. EPs are sub-micron-sized particles shed by all types of cells. They are free to travel to reach distant cells and can interact with them. Therefore they present an universal mechanism of cell-to-cell communication which is a subject of studies of different mechanisms underlying physiological and pathophysiological processes.

• Language: English
• Keywords: bioactive compounds, green product, microalgae biomass, microalgae sample preparation
• Typology: 1.08 - Published Scientific Conference Contribution
• Organization: ZF - Faculty of Health Sciences
• Year: 2025
• Number of pages: Str. 49-57
• PID: 20.500.12556/RUL-169995
• UDC: 502.131.1
• DOI: 10.55295/PSL.12.2025.III7
• COBISS.SI-ID: 240977667
• Publication date in RUL: 01.07.2025

Record is a part of a monograph

• Title: Socratic Lectures : 12th international symposium, Ljubljana, 11. 1. 2025
• Editors: Veronika Kralj-Iglič, Yelena Istileulova, Anna Romolo
• Place of publishing: Ljubljana
• Publisher: University of Ljubljana Press
• Year: 2025
• ISBN: 978-961-297-597-5
• COBISS.SI-ID: 237747715

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.