This diploma thesis explores the application of derivative spectroscopy to improve
spectral analysis in systems with overlapping UV-Vis absorbance peaks. The main
objective was to assess whether this technique could aid in identifying and differentiating
components in binary mixtures. To ensure reliable spectral data, scanning speed and data
smoothing methods were first optimized using a didymium glass filter, which provided
sharp, well-defined absorption features for evaluating spectrometer settings. This process
led to the selection of optimal measurement parameters.
UV-Vis spectra of individual substances ‒ trisodium citrate, HEPES, potassium
tetraoxalate, and potassium hydrogen phthalate ‒ were recorded and analyzed alongside
their corresponding binary mixtures with citrate. Comparisons of the first and second
derivatives revealed that, while derivative spectroscopy did not yield sufficiently clear
results for distinguishing components within mixtures, it proved valuable for improving
spectral resolution and accurately locating peak positions.
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