Performance evaluation of a novel MBT LipidART module (Bruker Daltonics) for detection of colistin resistance in Escherichia coli and Klebsiella pneumoniae

Germ, Julija (author), Pirš, Mateja (author)

We evaluated the MBT LipidART module on a MALDI Biotyper® Sirius System (Bruker Daltonics) for the rapid detection of colistin resistance in Escherichia coli (EC) and Klebsiella pneumoniae (KPN) by analysing lipid A profiles in negative ion mode. Categorical agreement was achieved for 98.3% EC (N = 58) and 85.0% KPN (N = 40). Challenges included calibration difficulties, limited availability of compatible equipment and issues with mucoid and adherent KPN isolates that yielded invalid results. While MBT LipidART module shows promise as a rapid tool for detection of colistin resistance, its performance was notably better for EC compared to KPN.

• Language: English
• Keywords: performance evaluation, MBT LipidART module, detection of colistin resistance, Escherichia coli, Klebsiella pneumoniae
• Work type: Article
• Typology: 1.03 - Other scientific articles
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: Str. 1501–1504
• Numbering: Vol. 44, iss. 6
• PID: 20.500.12556/RUL-169431
• UDC: 616-097:616.1
• ISSN on article: 0934-9723
• DOI: 10.1007/s10096-025-05099-4
• COBISS.SI-ID: 230357763
• Publication date in RUL: 28.05.2025

Record is a part of a journal

• Title: European journal of clinical microbiology & infectious diseases
• Shortened title: Eur. j. clin. microbiol. infect. dis.
• Publisher: Springer Nature
• ISSN: 0934-9723
• COBISS.SI-ID: 590356

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: ocena učinkovitosti, modul MBT LipidART, odkrivanje odpornosti na kolistin, Escherichia coli, Klebsiella pneumoniae