Vpliv BPC-157 na proliferacijo primarnih mezenhimskih matičnih/ stromalnih celic in vitro

Hohnjec, Karlina (author), Zupan, Janja (mentor), Haring, Gregor (comentor)

Dosedanje raziskave so dokazale, da BPC 157 pozitivno učinkuje pri celjenju različnih tkiv po travmatičnih in sistematskih poškodbah, vendar za uporabo na ljudeh še potrebujemo klinične študije. BPC 157 izboljša sposobnost hemostaze ter funkcijo žil, ima zaščitno vlogo za celice pri ulceroznem kolitisu in multipli sklerozi, zmanjša aritmije z različnimi vzroki nastanka, izboljša celjenje akutne in kronične poškodbe prebavnega trakta, kit in vezi, mišic, kosti, živcev, hrbtenjače ter ohrani prozornost roženice, boljša je tudi obnova živčno-mišičnega stika. Poškodbe, ki se same spontano ne bi mogle zaceliti, se ob dodatku BPC 157 zacelijo, poleg tega se celijo bolj kvalitetno. Študije do sedaj še niso odkrile negativnih stranskih učinkov, kar nakazuje na zelo varen profil BPC 157 z zelo dobrim regenerativnim učinkom. Namen magistrske naloge je bil ovrednotiti vpliv pentadekapeptida BPC 157 na sposobnost proliferacije primarnih mezenhimskih matičnih/ stromalnih celic (MSC) v pogojih in vitro. Vsi vzorci primarnih celic so bili izolirani iz kostnega mozga. Testirali smo sledeče hipoteze - tretiranje primarnih celic z BPC 157 spodbuja njihovo proliferacijo v pogojih in vitro; vpliv BPC 157 na sposobnost proliferacije MSC v pogojih in vitro je odvisen od koncentracije BPC 157 ter vpliv BPC 157 na sposobnost proliferacije MSC v pogojih in vitro je odvisen od časa izpostavljenosti. Uporabili smo gojišče DMEM z nizko vsebnostjo glukoze, fetalnim govejim serumom, penicilinom in streptamicinom. Celice smo tretirali z različnimi koncentracijami peptida BPC 157 od 250 μg/mL do 0,156 μg/mL. Sposobnost proliferacije celic smo ovrednotili s testom MTS po 24, 48 in 96 urah. Iz izmerjenih absorbanc po testu MTS, smo v programu R izvedli statistične analize. Rezultati so pokazali, da BPC 157 ne pospeši proliferacije celic MSC v pogojih in vitro ne glede na koncentracijo ali čas tretiranja, saj nismo dokazali statistično značilnih razlik med nobeno koncentracijo peptida in kontrolno skupino brez dodanega BPC 157. Dobro bi bilo preučiti še druge celične mehanizme, na katere morda vpliva preiskovani peptid ter zajeti večje število vzorcev, celice tretirati dalj časa in uporabiti večji razpon koncentracij BPC 157.

• Language: Slovenian
• Keywords: BPC 157, Proliferacija celic, Primarne mezenhimske matične/stromalne celice, Pogoji in vitro
• Work type: Master's thesis/paper
• Organization: FFA - Faculty of Pharmacy
• Year: 2025
• PID: 20.500.12556/RUL-169202
• Publication date in RUL: 17.05.2025

Secondary language

• Language: English
• Title: The effect of BPC-157 on proliferation of primary mesenchymal stem/ stromal cells in vitro

Abstract

Previous studies have demonstrated that BPC 157 has a positive effect on the healing of various tissues following traumatic and systemic injuries, but clinical trials are still needed to assess its use for humans. BPC 157 improves hemostasis and vascular function, provides a protective role for cells in ulcerative colitis and multiple sclerosis, reduces arrhythmias of various causes, enhances the healing of acute and chronic injuries to the gastrointestinal tract, tendons, ligaments, muscles, bones, nerves, and spinal cord, maintains corneal transparency and improves the recovery of neuromuscular junctions. Injuries that would not otherwise heal spontaneously are able to heal with the addition of BPC 157, and healing occurs with improved quality. To date, studies have not identified any negative side effects, suggesting a very safe profile for BPC 157 with excellent regenerative effects. The purpose of this research was to evaluate the effect of the pentadecapeptide BPC 157 on the proliferation capacity of primary mesenchymal stem/stromal cells (MSC) in in vitro conditions. All primary cell samples were isolated from bone marrow. We tested the following hypotheses: treatment of primary cells with BPC 157 stimulates their proliferation in vitro; the effect of BPC 157 on the proliferation of MSC in vitro depends on the concentration of BPC 157; and the effect of BPC 157 on the proliferation of MSC in vitro depends on the duration of exposure. We used DMEM medium with low glucose content, fetal bovine serum, penicillin, and streptomycin. The cells were treated with various concentrations of BPC 157 peptide, ranging from 250 μg/mL to 0.156 μg/mL. The cell proliferation capacity was assessed using the MTS assay at 24, 48, and 96 hours. Statistical analyses were performed in R software using the absorbance measurements obtained from the MTS assay. The results showed that BPC 157 does not accelerate the proliferation of MSCs in in vitro conditions, regardless of the concentration or treatment time, as no statistically significant differences were observed between any concentration of the peptide and the control group without BPC 157. It would be usefull to investigate other cellular mechanisms that the investigated peptide may affect, as well as to include a larger number of samples, treat the cells for a longer period, and use a wider range of BPC 157 concentrations.

• Keywords: BPC 157, Cell proliferation, Primary mesenchymal stem/ stromal cells, In vitro conditions