Blood transfusion is an established form of treatment based on the use of blood products from donated blood. After collection, the blood is separated into blood components such as erythrocytes, platelets and blood plasma and stored separately until use. During feeding, the quality of cells and blood plasma is checked using standardized laboratory procedures. The purpose of the master's thesis is to determine how the proportion of activated platelets and the concentration of released platelet extracellular vesicles change with the storage time of platelet preparations. As part of the task, the concentration of platelets, residual leukocytes, glucose and the proportion of activated platelets were measured in transfusion preparations during seven-day storage on the first, third and seventh day after blood donation. The number of extracellular vesicles was determined in two ways, namely by the nanoparticle tracking method and by imaging flow cytometry. The results showed a statistically significant decrease in glucose concentration and an increase in the proportion of activated platelets and extracellular vesicle concentrations with storage time, while there was no change in platelet, leukocyte or extracellular vesicle size. The measured concentrations were slightly higher with the nanoparticle tracking method than the results obtained by imaging flow cytometry, which confirms the need for further development and unification of methods for the analysis of extracellular vesicles. Given the findings that prolonged storage increases platelet activation and the concentration of released extracellular vesicles, further research could demonstrate the feasibility of monitoring platelet activation and release of platelet extracellular vesicles as an indicator of the quality of transfusion preparations.
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