The mutational landscape and functional effects of noncoding ultraconserved elements in human cancers

Bayraktar, Recep (author), Tang, Yitao (author), Dragomir, Mihnea P. (author), Kunej, Tanja (author)

The mutational landscape of phylogenetically ultraconserved elements (UCEs), especially those in noncoding DNAs (ncUCEs), and their functional relevance in cancers remain poorly characterized. Here, we perform a systematic analysis of whole-genome and in-house targeted UCE sequencing datasets from more than 3000 patients with cancer of 13,736 UCEs and demonstrate that ncUCE somatic alterations are common. Using a multiplexed CRISPR knockout screen in colorectal cancer cells, we show that the loss of several altered ncUCEs significantly affects cell proliferation. In-depth functional studies in vitro and in vivo further reveal that specific ncUCEs can be enhancers of tumor suppressors (such as ARID1B) and silencers of oncogenic proteins (such as RPS13). Moreover, several miRNAs located in ncUCEs are recurrently mutated. Mutations in miR-142 locus can affect the Drosha-mediated processing of precursor miRNAs, resulting in the down-regulation of the mature transcript. These results provide systematic evidence that specific ncUCEs play diverse regulatory roles in cancer.

• Language: English
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication version: Version of Record
• Year: 2025
• Number of pages: 17 str.
• Numbering: Vol. 11, iss. 8
• PID: 20.500.12556/RUL-168484
• UDC: 575:616
• ISSN on article: 2375-2548
• DOI: 10.1126/sciadv.ado2830
• COBISS.SI-ID: 226767107
• Publication date in RUL: 15.04.2025

Record is a part of a journal

• Title: Science advances
• Publisher: American Association for the Advancement of Science
• ISSN: 2375-2548
• COBISS.SI-ID: 523392025

Licences

• License: CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
• Link: http://creativecommons.org/licenses/by-nc/4.0/
• Description: A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.

Secondary language

• Language: Slovenian
• Keywords: genetika, medicina, bolezni, rak, UCE