Monitoring electroporation-induced changes in action potential generation in genetically engineered tet-on spiking HEK cells

Batista Napotnik, Tina (author), Kos, Bor (author), Rems, Lea (author)

Excitable cells such as neuronal and muscle cells can be primary targets in rapidly emerging electroporation-based treatments. However, they can be affected by electric pulses even in therapies where they are not the primary targets, and this can cause adverse side effects. Therefore, to optimize the electroporation-based treatments of excitable and non-excitable tissues, there is a need to study the effects of electric pulses on excitable cells, their ion channels, and excitability in vitro. For this purpose, a protocol was developed for optical monitoring of changes in action potential generation due to electroporation on a simple excitable cell model of genetically engineered tet-on spiking HEK cells. With the use of a fluorescent potentiometric dye, the changes in transmembrane voltage were monitored under a fluorescence microscope, and relevant parameters of cell responses were extracted automatically with a MATLAB application. This way, the excitable cell responses to different electric pulses and the interplay between excitation and electroporation could be efficiently evaluated.

• Language: English
• Keywords: electroporation, excitable cells, action potential
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Author Accepted Manuscript
• Year: 2024
• Number of pages: 12 str.
• Numbering: Vol. 211, art. e67257
• PID: 20.500.12556/RUL-168459
• UDC: 602.621
• ISSN on article: 1940-087X
• DOI: 10.3791/67257
• COBISS.SI-ID: 230333955
• Publication date in RUL: 14.04.2025

Record is a part of a journal

• Title: Journal of visualized experiments
• Shortened title: J. vis. exp.
• Publisher: MYJoVE Corporation
• ISSN: 1940-087X
• COBISS.SI-ID: 6867284

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: elektroporacija, vzdražne celice, akcijski potencial