Biophysics and electrophysiology of pulsed field ablation in normal and infarcted porcine cardiac ventricular tissue

Miklavčič, Damijan (author), Verma, Atul (author), Krahn, Philippa R. P. (author), Štublar, Jernej (author), Kos, Bor (author), Escartin, Terenz (author), Lombergar, Peter (author), Coulombe, Nicolas (author), Terricabras Casas, Maria (author), Jarm, Tomaž (author), Kranjc, Matej (author)

Pulsed Field Ablation (PFA) is a new ablation method being rapidly adopted for treatment of atrial fibrillation, which shows advantages in safety and efficiency over radiofrequency and cryo-ablation. In this study, we used an in vivo swine model (10 healthy and 5 with chronic myocardial infarct) for ventricular PFA, collecting intracardiac electrograms, electro-anatomical maps, native T1-weighted and late gadolinium enhancement MRI, gross pathology, and histology. We used 1000–1500V pulses, with 1–16 pulse trains to vary PFA dose. Lesions were assessed at 24 h, 7 days, and 6 weeks in healthy and at 48 h in infarcted ventricles. Comparisons of lesion sizes using a numerical model enabled us to determine lethal electric field thresholds for cardiac tissue and its dependence on the number of pulse trains. Similar thresholds were found in normal and infarcted hearts. Numerical modeling and temperature-sensitive MRI confirmed the nonthermal nature of PFA, with less than 2% of a lesion’s volume at the highest dose used being attributed to thermal damage. Longitudinal cardiac MRI and histology provide a comprehensive description of lesion maturation. Lesions shrink between 24 h and 7 days post-ablation and then remain stable out to 6 weeks post-ablation. Periprocedural electrograms analysis yields good correlation with lesion durability and size

• Language: English
• Keywords: ventricular arrhythmias, electroporation, pulsed filed ablation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 22 str.
• Numbering: 14, art. 32063
• PID: 20.500.12556/RUL-166863
• UDC: 004.93:616.13-007.64:537.635
• ISSN on article: 2045-2322
• DOI: 10.1038/s41598-024-83683-y
• COBISS.SI-ID: 221586947
• Publication date in RUL: 28.01.2025

MIKLAVČIČ, Damijan, VERMA, Atul, KRAHN, Philippa R. P., ŠTUBLAR, Jernej, KOS, Bor, ESCARTIN, Terenz, LOMBERGAR, Peter, COULOMBE, Nicolas, TERRICABRAS CASAS, Maria, JARM, Tomaž and KRANJC, Matej, 2024, Biophysics and electrophysiology of pulsed field ablation in normal and infarcted porcine cardiac ventricular tissue. Scientific reports [online]. 2024. Vol. 14,  32063. [Accessed 26 April 2025]. DOI 10.1038/s41598-024-83683-y. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=166863

Record is a part of a journal

• Title: Scientific reports
• Shortened title: Sci. rep.
• Publisher: Springer Nature
• ISSN: 2045-2322
• COBISS.SI-ID: 18727432

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: srčne aritmije, elektroporacija, katetrska ablacija