Advances in copper speciation, isotopic ratio measurement, bioimaging, and single-cell analysis : a critical review

Kozlica, Katarina (author), Milačič Ščančar, Radmila (author), Reberšek, Martina (author), Čemažar, Maja (author), Uršič Valentinuzzi, Katja (author), Ščančar, Janez (author)

Copper (Cu) is an essential trace element associated with various physiological processes in the human body. In humans, approximately 75–95% of Cu in plasma is tightly bound to ceruloplasmin (Cp). The remaining Cu (exchangeable Cu) is loosely bound to human serum albumin (HSA) and, to a lesser extent, to alpha-2-macroglobulin (α2M) and low molecular mass (LMM) amino acids. Neurodegenerative and neoplastic diseases may have a significant impact on Cu metabolism. In serum of Wilson's disease patients, the exchangeable Cu content is increased, while in patients with certain forms of cancer, elevated Cu-Cp levels are observed. Early diagnosis and treatment are crucial for the effective management of Cu-related disorders. This review critically evaluates analytical methods for Cu speciation, isotopic analysis, bioimaging, and single-cell analysis, which provide valuable information for the diagnosis and treatment of Cu-related disorders. Analytical challenges and future needs for the development of new methodological approaches are also addressed.

• Language: English
• Keywords: human samples, copper ceruloplasmin, copper human serum albumin, analytical methods, speciation, isotopic ratio measurement, bioimaging, single-cell analysis, review, copper
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: MF - Faculty of Medicine; BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: 24 str.
• Numbering: Vol. 184, art. 118152
• PID: 20.500.12556/RUL-166673
• UDC: 543
• ISSN on article: 1879-3142
• DOI: 10.1016/j.trac.2025.118152
• COBISS.SI-ID: 222568707
• Publication date in RUL: 21.01.2025

Record is a part of a journal

• Title: TrAC trends in analytical chemistry
• Shortened title: TrAC, Trends anal. chem.
• Publisher: Elsevier
• ISSN: 1879-3142
• COBISS.SI-ID: 23039493

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: človeški vzorci, analitične metode, pregledni članki, plazemski baker, merjenje izotopskega razmerja, speciacija, baker, homeostaza, patologija