Analysis of extractives in liquid and headspace samples of Silver fir using gas chromatography coupled with a mass selective detector

Vek, Viljem (author), Poljanšek, Ida (author), Osolnik, Urša (author), Oven, Primož (author)

The main objective of the study is to present a chromatographic method that allows rapid and accurate quality control of extractives in liquid and headspace samples of wood and bark of European silver fir (Abies alba Mill.). The chemical identity of silver fir extractives in liquid and headspace samples was investigated. Mature silver firs were harvested and samples of sapwood, heartwood, knotwood and bark were prepared. Liquid samples were obtained by solvent extraction at higher temperature and pressure, while the volatile extractives of silver fir tissues were collected by headspace sampling. The extractives in the liquid silver fir samples were silylated prior to chromatographic analysis. The samples were then measured using gas chromatography (GC) coupled with a mass selective detector (MSD). The samples were introduced into the GC-MSD system using the Automatic Liquid Sampler (ALS) and the Headspace Sampler (HS). A total of 55 compounds were detected in the silver fir wood and bark samples. The liquid samples consisted of a variety of carboxylic/dicarboxylic acids, sugars with sugar alcohols (inositols) and sugar acids, with citric acid, quininic acid and sucrose being the most frequently represented. The liquid bark samples contained mainly sugar-like compounds, while the knotwood extracts contained large amounts of phenolic compounds and lignans. D-pitinol was confirmed as the most characteristic GC-MSD peak of the silver fir extracts. Analysis of the headspace of silver fir revealed α-pinene, camphene, Dlimonene, β-myrcene, ocimene, 2-bornanone or D-camphor and α-terpineol as the characteristic monoterpenes. It was shown that the presented GC-MSD method is a suitable analytical tool for the chemical screening of low molecular weight compounds in both liquid and headspace samples of wood and bark. However, the analysis of higher molecular weight extractives requires a different analytical approach supported by other analytical methods such as LC/MS.

• Language: English
• Keywords: Abies alba Mill., wood, bark, extractives, headspace, polyphenols, gas chromatography-mass selective detection (GC-MSD)
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Publication date: 01.01.2024
• Year: 2024
• Number of pages: Str. 457-468
• Numbering: Vol. 75, iss. 4
• PID: 20.500.12556/RUL-166336
• UDC: 630*81
• ISSN on article: 0012-6772
• DOI: 10.5552/drvind.2024.0193
• COBISS.SI-ID: 221231875
• Publication date in RUL: 08.01.2025

Record is a part of a journal

• Title: Drvna industrija
• Shortened title: Drv. ind.
• Publisher: Sveučilište u Zagrebu, Fakultet šumarstva i drvne tehnologije = University of Zagreb, Faculty of Forestry and Wood Technology, Hrvatska komora inženjera šumarstva i drvne tehnologije = Croatian Chamber of Forestry and Wood Technology Engineers
• ISSN: 0012-6772
• COBISS.SI-ID: 2858754

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Croatian
• Title: Analiza tekuće i parne faze ekstraktivnih tvari jelovine primjenom plinske kromatografije sa spektrometrom masa
• Keywords: navadna jelka Abies alba Mill., les, skorja, ekstraktivi, polifenoli, plinska kromatografija, masna selektivna detekcija (GC-MSD)