Introduction: Decline of the somatosensory system function, which occurs with aging or diabetes, may influence balance control reactions. Less effective reactive balance contributes to an increased risk of falls, which is a significant concern for older adults and patients. Purpose: The aim was to determine the differences in balance, gait and responses to perturbation during walking between healthy adults and healthy older adults and between diabetic patients (with and without diabetic neuropathy) and healthy individuals. Methods: We included 19 healthy participants aged 50–70 and four patients with diabetes mellitus and diminished light touch sensation in their feet. We have tested light touch sensation on feet with monofilaments, measured static and dynamic balance (stabilometry, limits of stability, Mini balance evaluation systems test), gait speed and responses to medio-lateral perturbations during walking on treadmill (speed 1 m/s and 0.4 m/s). Results: There was larger ellipse area in stabilometry (firm surface, eyes closed) in the 60–69-year group compared to the 50–59-year group. Differences between the groups were noted only in responses to perturbations in the lateral direction during walking at a speed of 0.4 m/s. The stabilization response was more frequent in the 60–69-year group, while the jump response was more common in the 50–59-year group. The dominance of the lower limb in support was not associated with the response to perturbation. Only the patients with neuropathy exhibited poorer static and dynamic balance and slower speeds in comfortable and fast walking compared to healthy participants. During responses to perturbations while walking at 0.4 m/s, only the stepping reaction (intermediate step, crossover step) was present in both patients (without neuropathy), unlike in healthy participants. Conclusion: Consistent with previous studies, our results indicate poorer balance, slower walking speed, and different responses to lateral perturbations during walking at 0.4 m/s in the 60–69-year group compared to the 50–59-year group and in patients with diabetes compared to healthy individuals. Future research is recommended with a larger sample size and reduction of bias in interpreting responses to perturbations during walking.
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