Effect of combining CBD with standard breast cancer therapeutics

Dobovišek, Luka (author), Novak, Metka (author), Krstanović, Fran (author), Borštnar, Simona (author), Lah Turnšek, Tamara (author), Debeljak, Nataša (author)

Breast cancer is the most common malignancy in women worldwide. Sixty-five percent of breast cancers are estrogen and/or progesterone receptor positive. Estrogen receptor expression is a prognostic and predictive biomarker of response to endocrine therapy, which consists of the selective estrogen receptor modulator tamoxifen, aromatase inhibitors, and the selective estrogen receptor degrader fulvestrant. Cannabidiol is a phytocannabinoid that is emerging as a potential therapeutic agent. The aim of this study was to investigate the effect of cannabidiol on estrogen receptor-positive and estrogen receptor-negative representative breast cancer cell lines in combination with standard therapeutic agents used in clinical practice. To compare the effects of cannabidiol on breast cancer cell viability, cancer cell lines were exposed to increasing concentrations of cannabidiol. The effects of cannabidiol in combination with the endocrine therapeutics tamoxifen, fulvestrant, and the cyclin-dependent kinase inhibitor palbociclib on breast cancer cell viability were examined. We demonstrated that cannabidiol dose-dependently decreased the viability of all breast cancer cell lines independent of estrogen re- ceptor expression. The addition of cannabidiol to tamoxifen had an additive negative effect on cell viability in ER+ in estrogen receptor positive T-47D line. Cannabidiol did not attenuate the effect of standard treatment of hormone receptor-positive breast cancer with fulvestrant and palbociclib. In addition, cannabidiol did not attenuate the effect of standard treatment of triple-negative breast cancer and human epidermal growth factor receptor 2 positive breast cancer cell lines with trastuzumab and cisplatin.

• Language: English
• Keywords: breast cancer, cannabinoids, endocrine therapy, cannabidiol, CBD, cannabinoid receptor 1, CB1, cannabinoid receptor 2, CB2, hormone receptor, HR, palbociclib, cisplatin, trastuzumab, tamoxifen, fulvestrant
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine; FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 7 str.
• Numbering: Vol. 4, art. 100038
• PID: 20.500.12556/RUL-164995
• UDC: 616-006
• ISSN on article: 2667-3940
• DOI: 10.1016/j.adcanc.2022.100038
• COBISS.SI-ID: 104771331
• Publication date in RUL: 20.11.2024

Record is a part of a journal

• Title: Advances in cancer biology. Metastasis
• Publisher: Elsevier
• ISSN: 2667-3940
• COBISS.SI-ID: 87457795

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: rak dojke, kanabinoidi, endokrina terapija

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0390
• Name: Funkcijska genomika in biotehnologija za zdravje

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0245
• Name: Ekotoksiologija, toksikološka genomika in karcinogeneza