Identification of twenty-two new complete genome sequences of honeybee viruses detected in Apis mellifera carnica worker bees from Slovenia

Šimenc Kramar, Laura (author), Toplak, Ivan (author)

In this study, honeybee viruses were identified in naturally infected honeybee colonies (Apis mellifera carnica). From nine selected samples of clinically affected and ten samples of healthy honeybee colonies, different strains of honeybee viruses were first detected using quantitative real-time RT-PCR methods. Twenty-two nucleotide sequences of the complete genomes of honeybee viruses were identified using the Illumina Next-Generation Sequencing (NGS) method: acute bee paralysis virus (ABPV) (n = 4), black queen cell virus (BQCV) (n = 3), chronic bee paralysis virus (CBPV) (n = 2), deformed wing virus (DWV) (n = 5), Lake Sinai virus (LSV) (n = 4), sacbrood bee virus (SBV) (n = 1), Apis rhabdovirus-1 (ARV-1) (n = 1), bee macula-like virus (BeeMLV) (n = 1) and Hubei partiti-like virus 34 (HPLV34) (n = 1). The nucleotide sequences of ABPV, BQCV, DWV and SBV are the first complete genomes of these viruses identified in Slovenia and they represent an important contribution to our understanding of the genetic diversity of honeybee viruses. ARV-1, BeeMLV and HPLV34 were detected and sequenced for the first time in Slovenia.

• Language: English
• Keywords: honeybee viruses, next-generation sequencing, complete genomes, phylogeny, high-Ttroughput nucleotide sequencing, viruses, varroidae, bees
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 18 str.
• Numbering: Vol. 15, no. 11, art. 832
• PID: 20.500.12556/RUL-164411
• UDC: 578:636.09:638
• ISSN on article: 2075-4450
• DOI: 10.3390/insects15110832
• COBISS.SI-ID: 212838403
• Publication date in RUL: 24.10.2024

Record is a part of a journal

• Title: Insects
• Publisher: MDPI
• ISSN: 2075-4450
• COBISS.SI-ID: 519122457

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 24.10.2024

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana