Transactive response DNA binding protein 43 kDa, also known as TDP-43 is a representative of the heterogeneous nuclear ribonucleoprotein family (hnRNP). TDP-43 is an RNA- and DNA-binding protein that is under physiological conditions mainly located in the nucleus. There, it participates in the regulation of transcription and RNA metabolism, as well as many other processes, including the control of gene expression, miRNA processing, apoptosis, cell division and the formation of stress granules. It consists of N-terminal domain, two RNA-recognition motifs (RRM1 and RRM2) and C-terminal domain. In addition, it also contains a nuclear localization signal and a nuclear transport signal that enable shuttling of TDP-43 between the nucleus and the cytoplasm. Accumulation of TDP-43 protein aggregates and mislocalization are common pathological features in patients with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). We created deletion mutants of the TDP-43 protein using the IVA cloning method, which were then successfully expressed in Escherichia coli BL21[DE3] using autoinduction. Protein expression was examined with NaDS-PAGE and western blotting, whose results confirmed that we can continue the experimental work with isolation of the protein.
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