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Thermodynamic origin of the linear pressure dependence of DNA thermal stability
ID Lah, Jurij (Author), ID Hadži, San (Author)

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Abstract
High pressure affects the structure and function of DNA and is a key parameter for studying the origin and physical limits of life. Different types of DNA structures systematically show a linear pressure dependence of thermal stability (up to ∼200 MPa), which is maintained even when the solution composition is changed. The reasons behind the linear pressure dependence are not understood. We have performed a thermodynamic analysis of the pressure-, temperature- and composition-dependent (un)folding of various polynucleotide duplexes and G-quadruplexes. We demonstrate that the reason for the observed linearity is the link between compressibility and expansibility, both of which largely depend on DNA hydration. We predicted the temperature and pressure dependence of compressibility and expansibility of (un)folding and explain how they affect the corresponding volume change and thermodynamic stability parameters. These predictions indicate the existence of a convergence temperature at which compressibility and volume of (un)folding simultaneously become equal to zero.

Language:English
Keywords:biomolecules, DNA, G-quadruplex, stability, pressure, volume, compressibility, expansibility, biopolymers, genetics, hydration
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Publication status:Published
Publication version:Version of Record
Year:2024
Number of pages:Str. 9064-9069
Numbering:Vol. 15, iss. 35
PID:20.500.12556/RUL-161265-99fd15cd-a9db-9717-942c-1979723a1e93 This link opens in a new window
UDC:577.323
ISSN on article:1948-7185
DOI:10.1021/acs.jpclett.4c01563 This link opens in a new window
COBISS.SI-ID:205693699 This link opens in a new window
Publication date in RUL:09.09.2024
Views:162
Downloads:57
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Record is a part of a journal

Title:The journal of physical chemistry letters
Shortened title:J. phys. chem. lett.
Publisher:American Chemical Society
ISSN:1948-7185
COBISS.SI-ID:516049689 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:biomolekule, DNA, G-kvadrupleks, stabilnost, tlak, volumen, stisljivost, razteznost

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P1-0201
Name:Fizikalna kemija

Funder:ARRS - Slovenian Research Agency
Project number:J1-50026
Name:Vloga novega sekvenčnega motiva, bogatega z alanini, pri kondenzaciji RNA-vezavnih proteinov

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