Morfološka in genetska variabilnost oplutnika (Quercus crenata Lam.) v Sloveniji in srednji Italiji : diplomsko delo - univerzitetni študij

Rep, Ana (author), Brus, Robert (mentor), Simeone, Marco Cosimo (comentor)

Oplutnik (Quercus crenata Lam.) je predvsem zaradi svoje redkosti pri nas nepoznana vrsta. V Sloveniji so zabeleženi le trije primerki te vrste, dva na Krasu in eden v Halozah. Oplutnik naj bi bil križanec med cerom (Quercus cerris L.) in plutcem (Quercus suber L.). Namen naloge je bil preučiti morfološko in genetsko variabilnost oplutnika skupaj z domnevnimi starševskimi drevesi. Ker plutca pri nas ni, so ga poiskali v nam bližnji Hrvaški in ga smatrali kot potencialnega starša našim kraškim oplutnikom. Da bi vzorec povečali in ga s tem naredili smiselnega za analizo, so naša drevesa primerjali z drevesi v osrednji Italiji (provinca Viterbo). Morfološke analize so naredili po metodi Cristofolinija, genetske analize pa z restrikcijsko metodo. Rezultati so pokazali, da so si vrste zelo različne med seboj, pa tudi posamezni primerki znotraj vrste. Genetska razlika med haplotipi v isti populaciji je večja kot med populacijami. Pri morfometrijski analizi pa so ugotovili, da največ variabilnosti pojasnijo dolžina lista (LL), maksimalna širina lista (LW) ter razdalja od glavne listne žile do zavihka na točki maksimalne širine (LOB). V Sloveniji je ogrožen, zato so se posvetili tudi možnostim za ohranitev in naravno razmnoževanje oplutnika v slovenskih gozdovih.

• Language: Slovenian
• Keywords: Oplutnik, Quercus crenata, morfometrija, genske analize
• Work type: Undergraduate thesis
• Typology: 2.11 - Undergraduate Thesis
• Organization: BF - Biotechnical Faculty
• Place of publishing: Ljubljana
• Publisher: [A. Rep]
• Year: 2012
• Number of pages: VIII, 60 str.
• PID: 20.500.12556/RUL-16082
• UDC: 630*16
• COBISS.SI-ID: 3397798
• Publication date in RUL: 11.07.2014

Secondary language

• Language: Unknown

Abstract

Oplutnik is not a widely known species in Slovenia, mostly due to its rarity. There are only three registered specimens, two in the Karst region and one in Haloze. Oplutnik is believed to be a hybrid between Quercus cerris L. and Quercus suber L. The objective of this paper was to study and examine morphological and genetic variability of oplutnik with its putative parental species. Since Quercus suber L. does not grow on Slovenian territory, it was located it in nearby Croatia and treated it as a potential parental species to our Karstic Quercus crenata Lam. To enlarge the sample and make it relevant for the analysis, the comparation between Slovenia-grown trees and the trees from central Italy (the province of Viterbo) was done. Cristofolini method was applied for morphological analyses and restriction method for genetic analyses. The results showed high diversity between the species and also among individual specimens within a species. Genetic variability among haplotypes within the same population is higher than among different populations. Morphometric analysis showed that LL, LW and LOB explain most of the variation. Its existance is threatened in Slovenia, so there was done a list on the possibility to preserve and reproduct Quercus crenata Lam. in Slovenian forests.