Razvoj nanodelcev na osnovi hitosana in alginata z nizinom

Piškur, Vinona Čarna (author), Kocbek, Petra (mentor)

Polimerni nanodelci so dostavni sistemi, ki se uporabljajo za vgrajevanje tako zdravilnih učinkovin kot tudi kozmetično aktivnih sestavin. Z vgrajevanjem v polimerne nanodelce se poveča stabilnost vgrajene kozmetično aktivne sestavine, prav tako pa potencialno omogoča njeno podaljšano sproščanje, ciljana dostava in dostava v globje plasti kože kamor proste učinkovine težje prehajajo. Polimerni nanodelci so potencialni dostavni sistem tudi za peptidne kozmetično aktivne sestavine, kot je nizin. Nizin izkazuje protimikrobno delovanje in se v kozmetičnih izdelkih lahko uporablja kot konzervans. Mikroorganizmi na nizin težje razvijejo odpornost kot na številne druge protimikrobne sestavine, ne izkazuje celične citotoksičnosti in je na splošno priznan kot varen za uporabo. Sprva so ga uporabljali kot konzervans prehrambnih izdelkov, nato pa je sledila uporaba v biomedicini in v kozmetiki. Cilj diplomske naloge je bil izdelati polimerne nanodelce na osnovi hitosana in alginata z vgrajenim nizinom. Z optimizacijo pogojev izdelave in sestave formulacije smo izdelali prazne polimerne nanodelce s povprečno hidrodinamsko velikostjo delcev 670 nm in polidisperznim indeksom 0,54. V razvito formulacijo nanodelcev smo nato vgradili nizin. Povprečna hidrodinamska velikost delcev z nizinom je bila 641 nm in polidisperzni indeks 0,50. Fizikalno stabilnost tako praznih polimernih nanodelcev kot tudi polimernih nanodelcev z vgrajenim nizinom, smo spremljali do 8 tednov. Ugotovili smo, da prazni polimerni nanodelci v disperziji pri sobni temperaturi niso stabilni, saj se je njihova povprečna velikost povečala. V primeru polimernih nanodelcev z vgrajenim nizinom, ki smo jih shranjevali v hladilniku, pa se je povprečna velikost s časom zmanjševala in disperzija je postala manj polidisperzna. Ugotovili smo, da je za določanje nizina v disperzijah polimernih nanodelcev ustrezna metoda tekočinske kromatografije, ki je sklopljena z masno spektrometrijo, medtem ko metoda po Bradfordu ni ustrezna. Ugotovili smo tudi, da večanje količine nizina v disperziji nanodelcev ne vodi v večjo učinkovitost vgrajevanja nizina v nanodelce.

• Language: Slovenian
• Keywords: kozmetika, nanotehnologija, nizin, polimerni nanodelci, stabilnost
• Work type: Bachelor thesis/paper
• Organization: FFA - Faculty of Pharmacy
• Year: 2024
• PID: 20.500.12556/RUL-160722-468cfafb-9129-ca81-7932-7f17fd46e72d
• Publication date in RUL: 04.09.2024

Secondary language

• Language: English
• Title: Development of chitosan/alginate-based nanoparticles with nisin

Abstract

Polymeric nanoparticles are delivery systems used for encapsulating both medicinal compounds and cosmetic active ingredients. Encapsulation in polymeric nanoparticles increases the stability of the incorporated cosmetic active ingredient, and potentially allows for its prolonged release, targeted delivery, and delivery to deeper skin layers, which free compounds have more difficulty penetrating. Polymeric nanoparticles are a potential delivery system for peptide cosmetic active ingredients, such as nisin. Nisin exhibits antimicrobial activity and can be used as a preservative in cosmetic products. Microorganisms have more difficulty developing resistance to nisin compared to many other antimicrobial components. Nisin does not exhibit cellular cytotoxicity and is generally recognized as safe for use. Initially, it was used as a preservative in food products, followed by applications in biomedicine and cosmetics. The aim of the thesis was to create polymeric nanoparticles based on chitosan and alginate with incorporated nisin. By optimizing the production conditions and formulation composition, we produced empty polymeric nanoparticles with an average hydrodynamic size of 670 nm and polydispersity index 0,54. Nisin was then incorporated into the developed nanoparticle formulation. The average hydrodynamic size of the particles with nisin was 641 nm, and the polydispersity index was 0,50. We monitored the physical stability of both empty polymeric nanoparticles and polymeric nanoparticles with incorporated nisin for up to 8 weeks. We found that empty polymeric nanoparticles in dispersion were not stable at room temperature, as their average size increased. In contrast, polymeric nanoparticles with incorporated nisin, when stored in the refrigerator, showed a decrease in average size over time, and the dispersion became less polydisperse. We determined that liquid chromatography combined with mass spectrometry is the appropriate method for quantifying nisin in polymeric nanoparticle dispersions, whereas the Bradford method is not suitable. We also found that increasing the amount of nisin in the nanoparticle dispersion does not lead to greater efficiency in the incorporation of nisin into the nanoparticles.

• Keywords: cosmetics, nanotechnology, nisin, polymeric nanoparticles, stability