Cyanobacterial cyclic peptides can disrupt cytoskeleton organization in human astrocytes : a contribution to the understanding of the systemic toxicity of cyanotoxins

Bubik, Anja (author), Frangež, Robert (author), Žužek, Monika C. (author), Gutiérrez-Aguirre, Ion (author), Lah Turnšek, Tamara (author), Sedmak, Bojan (author)

The systemic toxicity of cyclic peptides produced by cyanobacteria (CCPs) is not yet completely understood. Apart from the most known damages to the liver and kidneys, symptoms of their neurotoxicity have also been reported. Hepatotoxic CCPs, like microcystins, as well as non-hepatotoxic anabaenopeptins and planktopeptins, all exhibit cytotoxic and cytostatic effects on mammalian cells. However, responses of different cell types to CCPs depend on their specific modes of interaction with cell membranes. This study demonstrates that non-hepatotoxic planktopeptin BL1125 and anabaenopeptins B and F, at concentrations up to 10 µM, affect normal and tumor human astrocytes (NHA and U87-GM) in vitro by their almost immediate insertion into the lipid monolayer. Like microcystin-LR (up to 1 µM), they inhibit Ser/Thr phosphatases and reorganize cytoskeletal elements, with modest effects on their gene expression. Based on the observed effects on intermediate filaments and intermediate filament linkage elements, their direct or indirect influence on tubulin cytoskeletons via post-translational modifications, we conclude that the basic mechanism of CCP toxicities is the induction of inter- and intracellular communication failure. The assessed inhibitory activity on Ser/Thr phosphatases is also crucial since the signal transduction cascades are modulated by phosphorylation/dephosphorylation processes.

• Language: English
• Keywords: astrocytes, cyclic cyanobacterial peptides, cytoskeletal organization, Ser/Thr phosphatases, systemic toxicity, peptides, cyanobacteria toxins
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 18 str.
• Numbering: Vol. 16, iss. 9, art. 374
• PID: 20.500.12556/RUL-160378
• UDC: 576:616-092
• ISSN on article: 2072-6651
• DOI: 10.3390/toxins16090374
• COBISS.SI-ID: 205320707
• Publication date in RUL: 27.08.2024

Record is a part of a journal

• Title: Toxins
• Shortened title: Toxins
• Publisher: MDPI
• ISSN: 2072-6651
• COBISS.SI-ID: 517594649

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 27.08.2024