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Cyanobacterial cyclic peptides can disrupt cytoskeleton organization in human astrocytes : a contribution to the understanding of the systemic toxicity of cyanotoxins
ID
Bubik, Anja
(
Author
),
ID
Frangež, Robert
(
Author
),
ID
Žužek, Monika C.
(
Author
),
ID
Gutiérrez-Aguirre, Ion
(
Author
),
ID
Lah Turnšek, Tamara
(
Author
),
ID
Sedmak, Bojan
(
Author
)
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MD5: F06BDF9A81CF5D83FDA1F35E2BFF4285
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https://www.mdpi.com/2072-6651/16/9/374
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Abstract
The systemic toxicity of cyclic peptides produced by cyanobacteria (CCPs) is not yet completely understood. Apart from the most known damages to the liver and kidneys, symptoms of their neurotoxicity have also been reported. Hepatotoxic CCPs, like microcystins, as well as non-hepatotoxic anabaenopeptins and planktopeptins, all exhibit cytotoxic and cytostatic effects on mammalian cells. However, responses of different cell types to CCPs depend on their specific modes of interaction with cell membranes. This study demonstrates that non-hepatotoxic planktopeptin BL1125 and anabaenopeptins B and F, at concentrations up to 10 µM, affect normal and tumor human astrocytes (NHA and U87-GM) in vitro by their almost immediate insertion into the lipid monolayer. Like microcystin-LR (up to 1 µM), they inhibit Ser/Thr phosphatases and reorganize cytoskeletal elements, with modest effects on their gene expression. Based on the observed effects on intermediate filaments and intermediate filament linkage elements, their direct or indirect influence on tubulin cytoskeletons via post-translational modifications, we conclude that the basic mechanism of CCP toxicities is the induction of inter- and intracellular communication failure. The assessed inhibitory activity on Ser/Thr phosphatases is also crucial since the signal transduction cascades are modulated by phosphorylation/dephosphorylation processes.
Language:
English
Keywords:
astrocytes
,
cyclic cyanobacterial peptides
,
cytoskeletal organization
,
Ser/Thr phosphatases
,
systemic toxicity
,
peptides
,
cyanobacteria toxins
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
VF - Veterinary Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2024
Number of pages:
18 str.
Numbering:
Vol. 16, iss. 9, art. 374
PID:
20.500.12556/RUL-160378
UDC:
576:616-092
ISSN on article:
2072-6651
DOI:
10.3390/toxins16090374
COBISS.SI-ID:
205320707
Publication date in RUL:
27.08.2024
Views:
54
Downloads:
16
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Title:
Toxins. Elektronski vir
Shortened title:
Toxins
Publisher:
MDPI
ISSN:
2072-6651
COBISS.SI-ID:
517594649
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
27.08.2024
Projects
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
J1-0848
Name:
Antikancerogeno delovanje bioaktivnih spojin cianobakterijskega izvora v nasprotju možganskih tumorjev - gliobastomov
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
P4-0053
Name:
Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih
Funder:
Other - Other funder or multiple funders
Funding programme:
Ministry of Defence, Administration for Civil Protection and Disaster Relief
Project number:
URSZR 4300-1117/2009/1
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