Chromatographic isolation of bacteriophages, especially for the application of phage
therapy, represents an extremely important aspect in terms of the safety and efficacy of
their use. The main aim of this thesis was to demonstrate the possibility of separating
bacteriophages based on a principle of ion-exchange chromatography, using polyHIPE
chromatographic supports. We cultivated bacteriophages, performed a preliminary
purification step with PEG, and chromatographically separated them from the bacterial
lysate using an HPLC system. We demonstrated that the separation of bacteriophages
T7 and ɸ6 using polyHIPE chromatographic supports is feasible. The strong anion
exchanger QA proved to be more effective in comparison to the weak anion exchanger
DEAE for the separation of both types of bacteriophages, primarily due to higher yields
and lower losses during the process. Comparing two bacteriophages, the
chromatography of phage T7 was found to be more efficient than isolation of phage ɸ6,
again due to higher yield. For the latter, an ineffective PEG precipitation process and
more difficult handling throughout the isolation process was noticed.
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