Molecular epidemiology and characterization of enteroviruses detected in cerebrospinal fluid and respiratory samples in Slovenia, 2014–2023

Berginc, Nataša (author), Lunar, Maja M. (author), Šramel, Nina (author), Poljak, Mario (author)

Enterovirus (EV) infections have various symptoms and severe complications, including death. To determine EV prevalence and EV types in Slovenia, data on over 25 000 EV RNA tests for diagnostics and surveillance from 2014 to 2023 were analyzed. Altogether, 3733 cerebrospinal fluid (CSF) and 21 297 respiratory (sentinel and clinical) samples were tested for EV RNA. EV typing was performed on all residual EV‐positive CSF samples and on subset of respiratory specimens. Altogether, 1238 samples tested positive for EV RNA: 238 (6.4%) CSF and 1000 (4.7%) respiratory samples. EV‐positive patients were predominantly male (p < 0.001). Many EV‐positive CSF samples were from infants under 3 months (33.1%), whereas most EV‐positive respiratory samples were from children 1 to 2 years old (49.2%). Echovirus 30 (E‐30) was most frequent in CSF (33.0%), followed by CV‐B5 (13.8%) and E‐6 (13.8%). CV‐A6 was most frequent in respiratory samples (16.0%), followed by EV‐D68 (7.6%) and CV‐A5 (7.4%). EV types in CSF and respiratory samples show diverse dynamics, with some outbreaks indicated. A significant difference was found in the EV detection rate between CSF and respiratory samples by age. Various EV types were characterized, showing that some EV types are more neurotropic or cause more severe infections.

• Language: English
• Keywords: cerebrospinal fluid, CSF, enteroviruses, respiratory samples, surveillance, typing
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 11 str.
• Numbering: Vol. 96, iss. 8, art. e29827
• PID: 20.500.12556/RUL-160182
• UDC: 616.9
• ISSN on article: 1096-9071
• DOI: 10.1002/jmv.29827
• COBISS.SI-ID: 203075587
• Publication date in RUL: 22.08.2024

Record is a part of a journal

• Title: Journal of medical virology
• Shortened title: J. med. virol.
• Publisher: Wiley
• ISSN: 1096-9071
• COBISS.SI-ID: 512820505

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: cerebrospinalna tekočina, enterovirusi, respiratorni vzorci, nadzor, tipizacija

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: University of Ljubljana, Faculty of Medicine, Institute of Microbiology and Immunology

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P3-0083
• Name: Odnosi parazitskega obstajanja