Roe deer is one of the most suitable animal species for the bioindication of environmental pollution. The following aims were emphasised: (i) determinationof heavy metals in the kidneys of 14 roe deer, shot in 2005 in Kozjansko region; (ii) the determination of heavy metals in the kidneys of 35 roe deer, shot in the same year in the Šalek Valley; (iii) the measurement of fluctuating asymmetry (FA) of 4 antlers traits of 163 roe deer, shot in the period from 1975 to 2005 in Kozjansko region. Levels of Pb, Hg and As were higher in Šalek Valley than in Kozjansko region, but the average levels of Cd in roe deer offspring kidneys were not significantly different. The maximum measured levels in Kozjansko region were even higher than in Šalek Valley and two samples were exceeding legal allowable measurment levels. For testing a reactive bioindication we used a method of measuring the fluctuating asymmetry (FA) of roe deer antlers. In temporal comparison of the period 1975 to 2005 we have discovered a change of the oscillating asymmetry, with its maximum in the late 80's and in the beggining of the 90's and its posterior downturn.