Elucidating the mechanisms of microbubble formation in intracardiac pulsed field ablation

Mahnič-Kalamiza, Samo (author), Miklavčič, Damijan (author), Lombergar, Peter (author), Mikuž, Blaž (author), Mattison, Lars M. (author), Sigg, Daniel C. (author), Kos, Bor (author)

Delivery of electrical energy for sensing or therapeutic purposes often involves electrochemical phenomena at the electrode-electrolyte solution interface. Release of gaseous bubbles that accompanies delivery of pulsed electric fields to tissues in applications such as electrochemotherapy of tumours and irreversible electroporation or pulsed field ablation in cardiac electrophysiology needs to be understood and characterized. We present an in vitro study using pulsed field delivery into saline, employing multiple different treatment protocols, two electrode geometries (pair of needles and a modified RF catheter), and two imaging systems to elucidate the complex relationship between the electrical treatment protocol, temperature changes at and around the electrodes, and gas release due to pulse delivery. Our primary objective was to identify the key parameters responsible for bubble formation and to highlight the importance of the treatment parameters and their interplay – ranging from the temperature to appropriate choice of electrode geometry, and, most importantly, to the choice of the treatment protocol. We found that bubbles originating from electrochemical reactions are more prevalent in monophasic pulsing protocols, whereas in high frequency biphasic pulsing protocols the bubbles are mainly caused by boiling of the medium. Degassing of liquid due to lower solubility of gasses at elevated temperatures does seems to play a role, though a minor one. We also observed that bubbles caused by boiling collapse very rapidly, whereas electrochemically produced bubbles or those produced through degassing appear to have longer lifetimes. Therefore, the treatment protocols most suited to minimizing gas release are biphasic trains of short (▫$\mu$▫s) pulses with a significant inter-pulse delay (i.e. low duty cycle) to prevent excessive heating. Moreover, electrodes must be designed to avoid high local current densities. Our findings have broad implications extending from lab-on-achip cell electroporation devices to intracorporeal pulsed field applications in the cardiovascular system, particularly pulsed field ablation procedures.

• Language: English
• Keywords: pulsed field ablation, gas release, electrochemistry, joule heating, water phase transition
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 12 str.
• Numbering: Vol. 497, ǂ[article no.] ǂ144550
• PID: 20.500.12556/RUL-159176
• UDC: 602.621:544.6:616-089.87
• ISSN on article: 1873-3859
• DOI: 10.1016/j.electacta.2024.144550
• COBISS.SI-ID: 200309251
• Publication date in RUL: 02.07.2024

Record is a part of a journal

• Title: Electrochimica acta
• Publisher: Elsevier
• ISSN: 1873-3859
• COBISS.SI-ID: 135479555

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: ablacija s pulznim električnim poljem, sproščanje plina, elektrokemija, joulovo segrevanje, fazni prehod vode

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P2-0249
• Name: Elektroporacija v biologiji, biotehnologiji in medicini

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P2-0026
• Name: Reaktorska tehnika