Effectiveness of Bradyrhizobium japonicum inoculation on nodulation dynamics in Glycine max (L.) roots

Miladinović, Vladimir (author), Popović, Vera (author), Delić, Dušica (author), Dajić Stevanović, Zora (author), Kiprovski, Biljana (author), Mikulič Petkovšek, Maja (author), Ugrenović, Vladan (author)

Bradyrhizobium japonicum bacteria on the intensity of nodulation during nine phenophases (R1-R7) in relation to the isoflavone content in soybean seeds. Nine domestic soybean varieties were sown in three replicates, with Bradyrhizobium japonicum inoculated and non-inoculated seeds. The different morphological characteristics of nodules, the number and mass of nodules were studied during plants’ ontogenesis. The content of individual isoflavones in the sowing seed material was determined using high performance liquid chromatography mass spectrometry (HPLC-MS). The most abundant isoflavone was acetylgenistin in the variety Galeb (2741.4 μg/g), and the highest content of all isoflavones was recorded in the variety Gorštak (8117.7 μg/g). The variety Sava of the inoculated treatment exhibited the highest average values of the nodule mass (21.1 mg) and the highest number of nodules (23.3) in the phenophase, in which 10% of pods reached full length (R4). The average values of mass and number of nodules were calculated for the entire vegetation period, being higher in the inoculated treatment. There was no positive correlation between the content of isoflavones in seeds and the number and mass of nodules. The impact of quantity of isoflavone on nodulation intensity in soybeans was not significant,which could be related to already saturated soil with nitrogen-fixing bacteria.

• Language: English
• Keywords: Bradyrhizobium japonicum, isoflavones, nitrogen fixation, nodule, soybean
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 17 str.
• Numbering: Vol. 52, Iss. 2, art. 13627
• PID: 20.500.12556/RUL-159122
• UDC: 631
• ISSN on article: 0255-965X
• DOI: 10.15835/nbha52213627
• COBISS.SI-ID: 200151299
• Publication date in RUL: 01.07.2024

Record is a part of a journal

• Title: Notulae botanicae Horti agrobotanici Cluj-Napoca
• Shortened title: Not. bot. Horti agrobot. Cluj-Napoca
• Publisher: Institutum agronomicum "Dr. Petru Groza", Hortus agrobotanicus
• ISSN: 0255-965X
• COBISS.SI-ID: 8610861

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: soja, inokulacija, Bradyrhizobium japonicum, fiksacija dušika, fenolne spojine, izoflavoni