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Inokulacija sejank obmorskega bora (Pinus Pinaster) z glivo Fusarium circinatum po tretiranju z metil jasmonatom : diplomsko delo - univerzitetni študij
ID Vrhovnik, Manca (Author), ID Jurc, Dušan (Mentor) More about this mentor... This link opens in a new window, ID Solla Hach, Alejandro (Co-mentor)

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Abstract
Z eksogeno aplikacijo hormona metilnega estra jasmonske kisline (MJ) je bil proučevan vpliv na odpornost sejank obmorskega bora (Pinus pinaster) proti patogeni glivi Fusarium circinatum. Ugotavljalo se je tudi pomen različnih koncentracij NPK (dušik, fosfor, kalij) gnojil za rast in razvoj sejank ter vpliv posameznih hranilnih elementov na odpornost sejank proti patogeni glivi.Bolezen borov smolasti rak je karantenska bolezen, ki kuži vse vrste borov, zlasti v drevesnicah in nasadih. Poskus je bil opravljen na 3 mesece starih sejankah Pinus pinaster. Sejanke so bile razdeljene v 4 bloke ter znotraj blokov v 9 skupin, z različnimi koncentracijami NPK gnojila. Dva bloka sejank (III in IV) sta bila najprej tretirana s 25 mM MJ, nato pa skozi rane okužena s patogeno glivo. Simptomi razvoja bolezni so bili opazovani 8 tednov. MJ je imel negativen vpliv na rast apikalnih meristemov in ni vplival na večjo odpornost sejank, smrtnost je bila celo večja kot pri netretiranih sejankah. Vzrok je lahko v toksičnem delovanju MJ. Razlike v količini travmatskih smolnih kanalov med tretiranimi in netretiranimi sejankami ni bilozaznati. Višje koncentracije gnojil v substratu vplivajo na hitrejšo in bujnejšo rast sejank. Dodani dušik je vplival na večjo občutljivost sejank za bolezen. Fosfor je povzročil močnejšo rast koreninskega sistema. Pri preučevanju vpliva kalija na sejanke, specifičnih sprememb nismo opazili.

Language:Slovenian
Keywords:Gibberella circinata, Fusarium subglutinans f. sp. pini, borov smolasti rak, jasmonska kislina, hormon, hranilni elementi, NPK, odpornost sejank, travmatski smolni kanali
Work type:Undergraduate thesis (m5)
Typology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Year:2010
Publisher:[M. Vrhovnik]
Number of pages:76 str. + 8 pril.
Place:Ljubljana
PID:20.500.12556/RUL-15906 This link opens in a new window
UDC:630*4
COBISS.SI-ID:3050150 This link opens in a new window
Publication date in RUL:11.07.2014
Views:926
Downloads:337
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Secondary language

Language:Unknown
Abstract:
Influence on the resistance toward the pathogen fungus Fusarium circinatum after exogenous application of methyl jasmonate (MJ) on seedlings of maritime pine (Pinus pinaster) was investigated. The impact of different concentrations of NPK (nitrogen, phosphorus, potassium) fertilizers on the growth and development of seedlings and consequently the effects of particular nutrients on the seedling resistance against the fungus was also examined. Pitch canker is a quarantine disease that can affect all species of pine, especially in nurseries and plantations. The experiment was performed on 3 months old seedlings of P. pinaster. The seedlings were divided into 4 blocks; each of them consisted of 9 groups with different NPK treatments. Seedlings in two blocks (III and IV) were treated with 25 mM of MJ. A day later, they were infected through wounds with F. circinatum. The symptoms weremonitored for 8 weeks. We have observed that MJ had no effect on better resistance; the mortality was even higher than in untreated seedlings. MJ had a negative influence on the growth of apical meristems. Possible cause could be the toxicity of MJ. There was no difference in the number of traumatic resin ducts between treated and untreated seedlings. Large concentrations of fertilizer in the substrate provoked the seedlings to grow faster and bigger, albeit lowering the resistance toward the fungus. The treatment with nitrogen especially lowered the plant resistance. Phosphorus caused stronger growth of the seedlings root system. No specific change was observed after treatment of plants with potassium.


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