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Whole genome sequencing and molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from patients with bacteraemia in Slovenia
ID
Dermota, Urška
(
Author
),
ID
Celar Šturm, Andraž
(
Author
),
ID
Triglav, Tina
(
Author
),
ID
Strašek, Katja
(
Author
),
ID
Velimirović, Ivana
(
Author
)
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https://link.springer.com/article/10.1007/s10096-024-04802-1
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Abstract
Purpose Data on the molecular epidemiology of methicillin-resistant Staphylococcus aureus isolates from patients with bacteraemia in Slovenia are lacking. The aim of this study was to phenotypically and genotypically investigate 82 MRSA strains isolated from patients with bloodstream infections in central Slovenia between 2019 and 2022. Methods Whole-genome sequencing of selected strains was performed to characterize the strains based on sequence typing, antimicrobial resistance, toxin, and virulence factors genes. Results Most MRSA carried SCCmec II (63.4%), followed by SCCmec IV (34.1%) and SCCmec V (2.5%). A high proportion of strains belonging to the ST225 lineage (45.1%) was observed, followed by ST97 (18.3%), ST2883 (15.9%), ST22 (9.8%), ST5 (3.7%), and the ST1, ST398 and ST45 lineages (2.4% each). Sixteen different spa types were identified, predominantly ST225-t003 (31.7%), ST97-t359 (15.9%), and ST2883-t4336 (14.6%). None of the strains carried Panton-Valentine leukocidin, exfoliative toxins, or toxic shock toxin. All MRSA strains were susceptible to linezolid, rifampicin, vancomycin, teicoplanin, and trimethoprim-sulfamethoxazole. MRSA strains were resistant to erythromycin, clindamycin, tetracycline and gentamicin, with a frequency of 74.4%, 74.4%, 8.5%, and 1.2%, respectively. Conclusion This study demonstrates that bacteraemia in central Slovenia is caused by diverse MRSA lineages. Identification of newly emerged lineages should be followed in the future to detect changes in the molecular epidemiology of MRSA in our country.
Language:
English
Keywords:
MRSA
,
bloodstream infections
,
whole genom sequencing
,
Slovenia
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2024
Number of pages:
Str. 969-977
Numbering:
Vol. 43, iss. 5
PID:
20.500.12556/RUL-158240
UDC:
616-097:616.1
ISSN on article:
0934-9723
DOI:
10.1007/s10096-024-04802-1
COBISS.SI-ID:
197289987
Publication date in RUL:
30.05.2024
Views:
173
Downloads:
31
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Record is a part of a journal
Title:
European journal of clinical microbiology & infectious diseases
Shortened title:
Eur. j. clin. microbiol. infect. dis.
Publisher:
Springer Nature
ISSN:
0934-9723
COBISS.SI-ID:
590356
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
okužbe krvnega obtoka
,
sekvenciranje celotnega genoma
,
Slovenija
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P3-0083
Name:
Odnosi parazitskega obstajanja
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