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Biolayer interferometry and its applications in drug discovery and development
ID Jug, Ana (Author), ID Bratkovič, Tomaž (Author), ID Ilaš, Janez (Author)

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Abstract
Biolayer interferometry (BLI) is an optical ‘dip-and-read’ biosensor method for real-time, label-free analysis of biomolecular interactions. It can be used for kinetic analyses, analyte detection and quantitation with mid-to high throughput. Here, we review how BLI can support diverse activities in the broad field of drug design and development, ranging from fragment and compound library screening, structure-activity relationship and selectivity analyses of small-molecule drug leads, to cell line development for the production of biopharmaceutics, optimization and surveillance of bioprocess, and characterization and quality control of biologic drug substances. We discuss the strengths and drawbacks of BLI and compare it to other well-established methods and techniques. With advances in sensitivity, BLI has established itself as a robust and versatile tool for interrogating molecular interactions and structures, expanding from the world of biological molecules to small synthetic compounds.

Language:English
Keywords:biosensor, drug discovery, library screening, label-free analysis, kinetics, affinity, biopharmaceutical development
Work type:Article
Typology:1.02 - Review Article
Organization:FFA - Faculty of Pharmacy
Publication status:Published
Publication version:Version of Record
Year:2024
Number of pages:13 str.
Numbering:Vol. 176, art. 117741
PID:20.500.12556/RUL-156335 This link opens in a new window
UDC:544.4:615.2
ISSN on article:0165-9936
DOI:10.1016/j.trac.2024.117741 This link opens in a new window
COBISS.SI-ID:194830851 This link opens in a new window
Publication date in RUL:20.05.2024
Views:337
Downloads:42
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Record is a part of a journal

Title:TrAC. Trends in analytical chemistry
Shortened title:TrAC, Trends anal. chem.
Publisher:Elsevier
ISSN:0165-9936
COBISS.SI-ID:26556928 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:biosenzorji, odkrivanje zdravil, knjižnica presejalnih pregledov, kinetika, analiza brez nalepk, afiniteta, biofarmacevtski razvoj

Projects

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0208
Name:Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0420
Name:Napredna imunološka zdravila in celični pristopi v farmaciji

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