Biolayer interferometry and its applications in drug discovery and development

Jug, Ana (author), Bratkovič, Tomaž (author), Ilaš, Janez (author)

Biolayer interferometry (BLI) is an optical ‘dip-and-read’ biosensor method for real-time, label-free analysis of biomolecular interactions. It can be used for kinetic analyses, analyte detection and quantitation with mid-to high throughput. Here, we review how BLI can support diverse activities in the broad field of drug design and development, ranging from fragment and compound library screening, structure-activity relationship and selectivity analyses of small-molecule drug leads, to cell line development for the production of biopharmaceutics, optimization and surveillance of bioprocess, and characterization and quality control of biologic drug substances. We discuss the strengths and drawbacks of BLI and compare it to other well-established methods and techniques. With advances in sensitivity, BLI has established itself as a robust and versatile tool for interrogating molecular interactions and structures, expanding from the world of biological molecules to small synthetic compounds.

• Language: English
• Keywords: biosensor, drug discovery, library screening, label-free analysis, kinetics, affinity, biopharmaceutical development
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 13 str.
• Numbering: Vol. 176, art. 117741
• PID: 20.500.12556/RUL-156335
• UDC: 544.4:615.2
• ISSN on article: 0165-9936
• DOI: 10.1016/j.trac.2024.117741
• COBISS.SI-ID: 194830851
• Publication date in RUL: 20.05.2024

Record is a part of a journal

• Title: TrAC. Trends in analytical chemistry
• Shortened title: TrAC, Trends anal. chem.
• Publisher: Elsevier
• ISSN: 0165-9936
• COBISS.SI-ID: 26556928

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: biosenzorji, odkrivanje zdravil, knjižnica presejalnih pregledov, kinetika, analiza brez nalepk, afiniteta, biofarmacevtski razvoj

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P1-0208
• Name: Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P1-0420
• Name: Napredna imunološka zdravila in celični pristopi v farmaciji