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Effects of electroporation on the function of sarco/endoplasmic reticulum ▫$Ca^{2+}$▫-ATPase and ▫$Na^+$▫,▫$K^+$▫-ATPase in H9c2 cells
ID Jan, Vid (Author), ID Jusović, Maida (Author), ID Miklavčič, Damijan (Author)

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Abstract
Pulsed field ablation (PFA) is a promising new treatment for atrial fibrillation (AF), in which pulmonary vein isolation is achieved by irreversible electroporation. Electroporation causes ATP to leak through the permeabilized membrane. ATP is required both for the healing of the cell membrane and for the functioning of ion pumps, such as sarco/endoplasmic reticulum ▫$Ca^{2+}$▫-ATPase (SERCA) or ▫$Na^+$▫,▫$K^+$▫-ATPase (NKA), which play a key role in maintaining continuous contractions of the heart muscle. We investigated the effects of electroporation on the expression of ion pumps and possible correlations with the activation of AMPK, the main energy sensor in cells. H9c2 rat cardiac cells were exposed to either monopolar or bipolar (H-FIRE) pulses. Cells lysed 4 or 24 h after electroporation were used for mRNA and protein expression analyses. Overall, both pulse protocols caused a dosedependent downregulation of crucial SERCA and NKA isoforms, except for NKA▫$\alpha$▫2 and ▫$\beta$▫3, which were upregulated after 24 h. Monopolar pulses also decreased the phosphorylation of FXYD1, which may cause an inhibition of NKA activity. Both pulse protocols caused an increased AMPK activity, which may decrease both SERCA and NKA activity via calcium/calmodulin-dependent protein kinase. Our results provide important new insights into what happens in surviving cardiomyocytes after they are exposed to PFA.

Language:English
Keywords:irreversible electroporation, reversible electroporation, pulsed field ablation, sarco/endoplasmic reticulum Ca2+-ATPase, Na+, K+-ATPase, atrial fibrillation, AMPK activation, H-FIRE pulses, H9c2 cells
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:FE - Faculty of Electrical Engineering
Publication status:Published
Publication version:Version of Record
Year:2024
Number of pages:21 str.
Numbering:Vol. 14, iss. 7 [article no.] 2695
PID:20.500.12556/RUL-156319 This link opens in a new window
UDC:602.621
ISSN on article:2076-3417
DOI:10.3390/app14072695 This link opens in a new window
COBISS.SI-ID:190227203 This link opens in a new window
Publication date in RUL:20.05.2024
Views:54
Downloads:17
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Record is a part of a journal

Title:Applied sciences
Shortened title:Appl. sci.
Publisher:MDPI
ISSN:2076-3417
COBISS.SI-ID:522979353 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:ireverzibilna ekektroporacija, reverzibilna elektroporacija, ablacija s pulznim električnim poljem, Ca2+-ATPaza sarko/endoplazemskega retikuluma, Na+, K+-ATPaza, atrijska fibrilacija, aktivacija AMPK, H-FIRE pulzi, celice H9c2

Projects

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P2-0249
Name:Elektroporacija v biologiji, biotehnologiji in medicini

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:I0-0022
Name:Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)

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