Your browser does not allow JavaScript!
JavaScript is necessary for the proper functioning of this website. Please enable JavaScript or use a modern browser.
Open Science Slovenia
Open Science
DiKUL
slv
|
eng
Search
Browse
New in RUL
About RUL
In numbers
Help
Sign in
Innovative approach for quantitative determination of ingested microplastics by Daphnia magna : use of diferential scanning calorimetry and thermogravimetry
ID
Procházková, Petra
(
Author
),
ID
Kalčíková, Gabriela
(
Author
),
ID
Maršálková, Eliška
(
Author
),
ID
Zlámalová Gargošová, Helena
(
Author
),
ID
Kučerík, Jiří
(
Author
)
PDF - Presentation file,
Download
(853,67 KB)
MD5: 8A4DFF86FE9276CAD7FC4B247A8459E0
URL - Source URL, Visit
https://link.springer.com/article/10.1007/s10973-024-12985-0
Image galllery
Abstract
Due to the serious environmental impacts of plastic pollution, some conventional plastics have been replaced with biodegradable alternatives. However, these biodegradable options can also fragment and form microplastics, which can be ingested by and harm various biota. The standard methods for quantifying ingested microplastics involve digestion of the gut or the whole organism using acidic, alkaline, or oxidative processes. However, these aggressive methods may destroy biodegradable microplastics, leading to erroneous results. Therefore, in this study, we employed thermal analysis methods, specifically differential scanning calorimetry (DSC) and thermogravimetry (TG), to quantify the ingestion of poly-3-hydroxybutyrate (P3HB), a biodegradable microplastic, by the freshwater crustacean, Daphnia magna. During chronic experiments, we analysed organisms exposed to P3HB with sizes smaller than 125 and 63 μm, at concentrations ranging from 1.56–25 mg L$^{−1}$. DSC identified an endothermic peak associated with the melting of semicrystalline P3HB, and its enthalpies were utilised to compute the number/mass of P3HB ingested by D. magna. Notably, shifts in melting points suggested that higher concentrations induced particle agglomeration, and these agglomerates could not penetrate deeply into the organism. The TG approach involved subtracting the mass loss between 200 and 400 °C in D. magna specimens exposed to P3HB suspensions from controls without P3HB exposure. Both methods provided comparable data, revealing that, depending on particle size, individual D. magna ingested up to 10% of their body mass. Our findings indicate that both methods effectively detect P3HB (and potentially other plastic fragments), with DSC demonstrating better sensitivity. While the suggested approach did not enable us to calculate the level of determination or quantification, we were able to demonstrate that DSC can detect P3HB in only one specimen of D. magna exposed to the lowest suspension concentration. This indicates that D. magna exposed to the 63 μm fraction ingested approximately 3 μg of P3HB, whereas those exposed to the 125 μm P3HB ingested around 4 μg of P3HB. The introduced methods expand the possibilities for detecting ingested microplastics and probably also nanoplastics, in zooplankton and possibly also other species.
Language:
English
Keywords:
microplastics
,
Daphnia magna
,
poly(3-hydroxybutyrate)
,
biodegradable plastics
,
ingestion
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
FKKT - Faculty of Chemistry and Chemical Technology
Publication status:
Published
Publication version:
Version of Record
Year:
2024
Number of pages:
8 str.
Numbering:
Vol. , iss.
PID:
20.500.12556/RUL-155752
UDC:
504.5:678:543.57
ISSN on article:
1388-6150
DOI:
10.1007/s10973-024-12985-0
COBISS.SI-ID:
192157443
Publication date in RUL:
15.04.2024
Views:
322
Downloads:
40
Metadata:
Cite this work
Plain text
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Copy citation
Share:
Record is a part of a journal
Title:
Journal of thermal analysis and calorimetry
Shortened title:
J. therm. anal. calorim.
Publisher:
Springer
ISSN:
1388-6150
COBISS.SI-ID:
13803815
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
mikroplastika
,
Daphnia magna
,
poli(3-hidroksibutirat)
,
biorazgradljiva plastika
Projects
Funder:
Other - Other funder or multiple funders
Project number:
FCH-S-23- 8297
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
P2-0191
Name:
Kemijsko inženirstvo
Similar documents
Similar works from RUL:
Similar works from other Slovenian collections:
Back