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Amyloid fibrils of stefin B show anisotropic properties
ID Žganec, Matjaž (Author), ID Taler-Verčič, Ajda (Author), ID Muševič, Igor (Author), ID Škarabot, Miha (Author), ID Žerovnik, Eva (Author)

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Abstract
Human stefin B, a member of the cystatin family of cysteine protease inhibitors, tends to form amyloid fibrils under relatively mild conditions, which is why it is used as a model protein to study amyloid fibrillation. Here, we show for the first time that bundles of amyloid fibrils, i.e., helically twisted ribbons, formed by human stefin B exhibit birefringence. This physical property is commonly observed in amyloid fibrils when stained with Congo red. However, we show that the fibrils arrange in regular anisotropic arrays and no staining is required. They share this property with anisotropic protein crystals, structured protein arrays such as tubulin and myosin, and other anisotropic elongated materials, such as textile fibres and liquid crystals. In certain macroscopic arrangements of amyloid fibrils, not only birefringence is observed, but also enhanced emission of intrinsic fluorescence, implying a possibility to detect amyloid fibrils with no labels by using optical microscopy. In our case, no enhancement of intrinsic tyrosine fluorescence was observed at 303 nm; instead, an additional fluorescence emission peak appeared at 425 to 430 nm. We believe that both phenomena, birefringence and fluorescence emission in the deep blue, should be further explored with this and other amyloidogenic proteins. This may allow the development of label-free detection methods for amyloid fibrils of different origins.

Language:English
Keywords:nano-sized object, bundle of amyloid fibrils, birefringence, intrinsic fluorescence, label-free imaging
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:FMF - Faculty of Mathematics and Physics
MF - Faculty of Medicine
Publication status:Published
Publication version:Version of Record
Year:2023
Number of pages:10 str.
Numbering:Vol. 24, iss. 4, art. 3737
PID:20.500.12556/RUL-155598 This link opens in a new window
UDC:577.112
ISSN on article:1661-6596
DOI:10.3390/ijms24043737 This link opens in a new window
COBISS.SI-ID:142086659 This link opens in a new window
Publication date in RUL:08.04.2024
Views:194
Downloads:66
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Record is a part of a journal

Title:International journal of molecular sciences
Shortened title:Int. j. mol. sci.
Publisher:MDPI
ISSN:1661-6596
COBISS.SI-ID:36217605 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:delci nanovelikosti, skupki amiloidnih fibril, dvolomnost, intrinzična fluorescenca, slikanje brez označevanja

Projects

Funder:ARRS - Slovenian Research Agency
Project number:J7-4050
Name:Oligomeri amiloidogenih proteinov od a do ž; biofizikalne lastnosti, struktura, funkcija in medsebojne interakcije

Funder:ARRS - Slovenian Research Agency
Project number:P1-0140
Name:Proteoliza in njena regulacija pri zdravju in boleznih

Funder:EC - European Commission
Funding programme:PhD program

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