Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian cattle

Ristanić, Marko (author), Zorc, Minja (author), Glavinić, Uroš (author), Stevanović, Jevrosima (author), Blagojevic, Jovan (author), Maletić, Milan (author), Stanimirović, Zoran (author)

To improve the genomic evaluation of milk-related traits in Holstein-Friesian (HF) cattle it is essential to identify the associated candidate genes. Novel SNP-based analyses, such as the genetic mapping of inherited diseases, GWAS, and genomic selection, have led to a new era of research. The aim of this study was to analyze the association of each individual SNP in Serbian HF cattle with milk production traits and inbreeding levels. The SNP 60 K chip Axiom Bovine BovMDv3 was deployed for the genotyping of 334 HF cows. The obtained genomic results, together with the collected phenotypic data, were used for a GWAS. Moreover, the identification of ROH segments was performed and served for inbreeding coefficient evaluation and ROH island detection. Using a GWAS, a polymorphism, rs110619097 (located in the intron of the CTNNA3 gene), was detected to be significantly (p < 0.01) associated with the milk protein concentration in the first lactation (adjusted to 305 days). The average genomic inbreeding value (FROH) was 0.079. ROH islands were discovered in proximity to genes associated with milk production traits and genomic regions under selection pressure for other economically important traits of dairy cattle. The findings of this pilot study provide useful information for a better understanding of the genetic architecture of milk production traits in Serbian HF dairy cows and can be used to improve lactation performances in Serbian HF cattle breeding programs.

• Language: English
• Keywords: GWAS, ROH, FROH, inbreeding, milk production, cattle, Holstein-Friesian, milk proteins
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 13 str.
• Numbering: Vol. 14, no. 5, art. 669
• PID: 20.500.12556/RUL-154574
• UDC: 636.2:637.5
• ISSN on article: 2076-2615
• DOI: 10.3390/ani14050669
• COBISS.SI-ID: 186289923
• Publication date in RUL: 21.02.2024

Record is a part of a journal

• Title: Animals
• Shortened title: Animals
• Publisher: MDPI AG
• ISSN: 2076-2615
• COBISS.SI-ID: 519120409

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: govedo, krave, molznice, mleko, prireja, lastnosti mlečnosti, genetika, genomika, selekcija, pasme, holštajn frizijsko govedo, Srbija

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: IAEA - International Atomic Energy Agency
• Project number: Research Contract 20774
• Name: Animal Identification, Pedigree, Exterior and Performance Data Recording in Selected Holstein-Friesian (HF) Cattle Population in Serbia Used for Future Genetic Selection under AI Programmes

• Funder: Other - Other funder or multiple funders
• Funding programme: Ministry of Science, Technological Development and Innovation of the Republic of Serbia
• Project number: Contract No. 451-03-47/2023-01/200143