Effect of hydroxypropyl methylcellulose (HPMC) hard capsules on in vitro rumen fermentation kinetics of commonly used feeds in ruminant feeding

Valcl, Nejc (author), Lavrenčič, Andrej (author)

Background Accurate dosing of feed additives is often required to evaluate their effects on rumen fermentation. This can be done using soluble but nonfermentable hydroxypropyl methylcellulose (HPMC) hard capsules. Objectives The aim of the study was to evaluate the effect of HPMC hard capsules on the extent and rate of in vitro gas production in eleven feeds. Methods Six high-fibre feeds and five concentrates, were weighed into syringes either directly or into HPMC capsules and incubated anaerobically in 30 mL buffered rumen fluid at 39°C. Data obtained from gas production measurements were fitted using the Gompertz model to obtain kinetic parameters for gas production. Results HPMC hard capsules had no effect on the gas production of the blank sample and concentrate feeds. In contrast, high-fibre feeds weighed in HPMC showed a significant decrease (p < 0.05) in total gas production and gas produced within 24 h of incubation. Conclusions The use of HPMC hard capsules was found to be inappropriate for determining gas production kinetics because fermentation subsides at a certain point when peak fermentation is reached (at TMFR), resulting in a decrease in both total potential gas production and gas production within 24 h of incubation. This is particularly evident when high-fibre feeds are incubated.

• Language: English
• Keywords: feeds, hard capsules, hydroxypropyl methylcellulose, in vitro gas production, ruminants
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 6 str.
• Numbering: Vol. 10, iss. 2, art. e1349
• PID: 20.500.12556/RUL-154547
• UDC: 636.084/.087
• ISSN on article: 2053-1095
• DOI: 10.1002/vms3.1349
• COBISS.SI-ID: 185932035
• Publication date in RUL: 20.02.2024

Record is a part of a journal

• Title: Veterinary medicine and science
• Shortened title: Vet. med. sci.
• Publisher: J. Wiley & Sons
• ISSN: 2053-1095
• COBISS.SI-ID: 525255961

Licences

• License: CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
• Link: http://creativecommons.org/licenses/by-nc/4.0/
• Description: A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.

Secondary language

• Language: Slovenian
• Keywords: prehrana živali, vamp, in vitro fermantacija, tvorba plinov, krma, hidroksipropilmetil celuloza, trde kapsule