CRISPR technologies in Chinese hamster ovary cell line engineering

Glinšek, Katja (author), Bozovičar, Krištof (author), Bratkovič, Tomaž (author)

The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of CHO cell lines for improved protein production and enhanced product quality. This review summarizes recent advances in the application of CRISPR technology for CHO cell line engineering with a particular focus on glycosylation modulation, productivity enhancement, tackling adventitious agents, elimination of problematic host cell proteins, development of antibiotic-free selection systems, site-specific transgene integration, and CRISPR-mediated gene activation and repression. The review highlights the potential of CRISPR technology in CHO cell line genome editing and epigenetic engineering for the more efficient and cost-effective development of biopharmaceuticals while ensuring the safety and quality of the final product.

• Language: English
• Keywords: Chinese hamster ovary cells, cell line development, CRISPR-Cas, genome editing, glycoengineering, augmenting productivity
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 26 str.
• Numbering: Vol. 24, iss. 9, art. 8144
• PID: 20.500.12556/RUL-154103
• UDC: 575.111
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms24098144
• COBISS.SI-ID: 150745347
• Publication date in RUL: 25.01.2024

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: jajčne celice kitajskega hrčka, razvoj celične linije, urejanje genoma, glikoinženiring, enocelično zaporedje, povečanje produktivnosti, genomika

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0127
• Name: Farmacevtska biotehnologija: znanost za zdravje