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Primerjava kemiluminiscenčne in encimske imunokemijske metode za določanje galektina-3 v serumu
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Trošt Lah, Eva
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Marc, Janja
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Žarak, Marko
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Abstract
Galektin-3 je protein, ki sodeluje pri vnetju in fibrozi, ter tako igra ključno vlogo pri razvoju in napredovanju srčnega popuščanja, ki je glavni vzrok obolevnosti in umrljivosti v Evropi. Zgodnje odkrivanje srčnega popuščanja je bistvenega pomena za njegovo hitro in učinkovito zdravljenje. Medtem ko se trenutni biomarkerji sproščajo po nastopu patofiziološkega procesa, galektin-3 kaže potencial kot zgodnji pokazatelj sprememb srčne mišice, zaradi česar je obetaven srčni biomarker za zgodnje odkrivanje bolezni. V magistrski nalogi smo primerjali kemiluminiscentno imunokemijsko metodo na mikrodelcih (CMIA) z encimsko imunsko metodo (ELISA) za določanje galektina-3. V raziskavo smo vključili 150 preiskovancev, od tega 84 žensk ( x̄ = 59 let) in 66 moških (x̄ = 58 let), brez prisotnosti srčne patološke spremembe. Rezultati naše študije so pokazali, da metodi ELISA in CMIA zaradi uporabe različnih protiteles nista zamenljivi (Passing-Bablokova regresijska analiza je ugotovila statistično pomembno proporcionalno in sistematično napako v rezultatih). Izmerjeni rezultati CMIA so bili v povprečju višji za 57 % od meritev ELISA. Spol in starost nista bistveno vplivala na koncentracije galektina-3 v serumu, vendar pa je metoda CMIA pokazala višje koncentracije pri ženskem spolu (mediana = 15,05 ng/mL) v primerjavi z moškim spolom (mediana = 13,40 ng/mL) za 12 %. Podobno je bilo opaziti tudi pri vplivu starosti, kjer so bile koncentracije galektina-3 pri osebah starejših od 60 let višje (mediana = 15,50 ng/mL), kot pri osebah maljših od 60 let (mediana = 12,70 ng/mL) za 22 %. Hitrost glomerulne filtracije je pomembno vplivala na koncentracije galektina-3, pri čemer bi bili za posameznike z oslabljeno ledvično funkcijo (eGFR < 90 mL/min/1,73 m²; mediana: 15,30 ng/mL) potrebni različni referenčni intervali, kot za osebe z normalno ledvično funkcijo (eGFR ⡥ 90 mL/min/1,73 m²; mediana: 13,20 ng/mL). V magistrski nalogi smo prav tako pokazali, da sta galektina-3 in marker srčnega popuščanja NT-proBNP komplementarna in ne zamenljiva indikatorja. Vključitev galektina-3 v klinično prakso skupaj z NT-proBNP bi lahko izboljšala diagnozo srčnega popuščanja in olajšala zgodnje odkrivanje, kar bi vodilo do pravočasnega preventivnega zdravljenja
Language:
Slovenian
Keywords:
galektin-3
,
srčno popuščanje
,
kemiluminiscenčni imunski test z mikrodelci
,
encimsko imunski test
,
referenčni interval
Work type:
Master's thesis/paper
Organization:
FFA - Faculty of Pharmacy
Year:
2023
PID:
20.500.12556/RUL-152917
Publication date in RUL:
12.12.2023
Views:
331
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49
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Language:
English
Title:
Comparison of chemiluminescent and enzyme immunochemical method for the determination of galectin-3 in serum
Abstract:
Galectin-3 is a protein that plays a crucial role in the development and progression of heart failure, which is the leading cause of morbidity and mortality in Europe. Early detection of heart failure is essential for prompt and effective treatment. While current biomarkers are released after the onset of the pathophysiological process, galectin-3 shows potential as an early indicator of cardiac muscle changes, making it a promising cardiac biomarker for early disease detection. A study compared the chemiluminescence method on micro-particles (CMIA) with the enzyme immunoassay method (ELISA) for galectin-3 determination. The study included 150 participants without any cardiac pathological changes, with 84 females (mean age = 59 years) and 66 males (mean age = 58 years). The results of the study showed that the ELISA and CMIA methods are not interchangeable due to the use of different antibodies (Passing-Bablok regression analysis revealed statistically significant proportional and systematic error in the results). The CMIA measurements were on average 57% higher than the ELISA measurements. Gender and age did not significantly affect serum galectin-3 concentrations. However, the CMIA method showed higher concentrations in females (median = 15.05 ng/mL) compared to males (median = 13.40 ng/mL) by 12%. Similarly, higher galectin-3 concentrations were observed in individuals older than 60 years (median = 15.50 ng/mL) compared to those younger than 60 years (median = 12.70 ng/mL) by 22%. The rate of glomerular filtration had a significant impact on galectin-3 concentrations. Different reference intervals would be needed for individuals with impaired renal function (eGFR < 90 mL/min/1.73 m²; median: 15.30 ng/mL) compared to those with normal renal function (eGFR ⡥ 90 mL/min/1.73 m²; median: 13.20 ng/mL).Furthermore, the study demonstrated that galectin-3 and the heart failure marker NT-proBNP are complementary indicators rather than interchangeable. Incorporating galectin-3 alongside NT-proBNP could improve the diagnosis of heart failure and facilitate early detection, leading to timely preventive treatment.
Keywords:
galectin-3
,
heart failure
,
chemiluminescent microparticle immunoassay
,
enzyme-linked immunosorbent assay
,
reference interval
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