Campylobacter magnus sp. nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus)

Gruntar, Igor (author), Kostanjšek, Rok (author), Pirš, Tina (author), Papić, Bojan (author)

During the 2021 European Food Safety Authority coordinated harmonized monitoring of antimicrobial resistance in Campylobacter species in Slovenia, five Campylobacter-like strains were cultured from caeca of a total of 104 domestic pigs that could not be identified using the standard-prescribed biochemical tests or MALDI-TOF MS. The isolates were obtained using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with prolonged cultivation time. Small Campylobacter like colonies were observed on mCCDA and CASA agar plates after 2–4days of incubation; dark-field microscopy revealed relatively big spirilli-shaped bacteria exhibiting characteristic Campylobacter-like motility. The cells were 1.5–3µm long and 0.5–0.7µm wide, Gram-negative, oxidase-positive and catalase-positive. MALDI-TOF mass spectra were distinctive and consistent, but with low MALDI-TOF MS log scores and the closest matches being those of Campylobacter hyointestinalis and Campylobacter fetus. All five strains underwent whole-genome sequencing. Analysis of 16S rRNA gene sequences revealed that the isolates were most similar (98.3–98.4% identity) to Campylobacter lanienae. Pairwise average nucleotide identity (ANI) values revealed that the five studied strains shared pairwise ANI of 96.2–96.5% but were clearly distinct from the previously described Campylobacter species (ANI ≤72.8%). The core genome-based phylogeny confirmed that the new strains form a distinct and well-supported clade within the genus Campylobacter. The conducted polyphasic taxonomic analysis confirmed that the five strains represent a novel Campylobacter species for which the name Campylobacter magnus sp. nov. is suggested, with strain 46386T (=DSM 115534T =CCUG 76865T ) as the type strain.

• Language: English
• Keywords: Campylobacter infections, swine
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Publication date: 01.01.2023
• Year: 2023
• Number of pages: Str. 1-7
• Numbering: Vol. 73, iss. 10, [article no.] 006108
• PID: 20.500.12556/RUL-152073
• UDC: 636.09:616.9:579
• ISSN on article: 1466-5034
• DOI: 10.1099/ijsem.0.006108
• COBISS.SI-ID: 170365443
• Publication date in RUL: 30.10.2023

Record is a part of a journal

• Title: International journal of systematic and evolutionary microbiology
• Shortened title: Int. j. syst. evol. microbiol.
• Publisher: Microbiology Society
• ISSN: 1466-5034
• COBISS.SI-ID: 521767193

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana