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Koncentracija cinka, kadmija in svinca v koreninah, listih, cvetovih in cvetnem ekstraktu navadne konoplje (Cannabis sativa), pridelane v Celjski kotlini
ID Gerlušnik, Anej (Author), ID Flajšman, Marko (Mentor) More about this mentor... This link opens in a new window, ID Pongrac, Paula (Co-mentor)

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Abstract
Težke kovine kot so cink (Zn), kadmij (Cd) in svinec (Pb) se v tleh pogosto pojavljajo v prekomernih koncentracijah. Namen raziskave je bil oceniti prenos Zn, Cd in Pb po konoplji, ki smo jo pridelali v Celjski kotlini v tleh s povečanimi koncentracijami Zn, Cd in Pb. Bločni poljski poskus s sadikami smo postavili poleti 2021, in sicer 4 sorte industrijske konoplje z evropske sortne liste (Carmagnola Selected, Elleta Campana, Finola in Tiborszallasi) in 4 odmerke zeolita (0 kg, 0,1 kg, 0,5 kg in 1 kg), ki smo ga dodali v sadilno luknjo pred sajenjem sadik. Ob zrelosti ženskih rastlin smo rastline povzorčili in v koreninah, listih in socvetju ter v cvetnem esktraktu izmerili koncentracije Zn, Cd in Pb. Največje koncentracije Zn smo izmerili v listih (55,6- 254,6 mg/kg v suhi snovi (SS)), nato v socvetju (74,6-182,5 mg/kg SS) in nazadnje v koreninah (52,1-93,9 mg/kg SS). Največje koncentracije Cd smo izmerili v koreninah (0,7-1,4 mg/kg SS), nato v socvetju (0,2-0,5 mg/kg SS) in najmanjše v listih (0,15-0,45 mg/kg SS). Največje koncentracije Pb smo izmerili v koreninah (3,4- 6,2 mg/kg SS), nato v listih (0,49-1,99 mg/kg SS), najmanjše koncentracije pa v socvetjih (0,3-1 mg/kg SS). Različni odmerki zeolita niso statistično značilno vplivali na koncentracije Zn, Cd in Pb, razen pri koncentraciji Pb v socvetju in še tam zelo blago (obstajala je le statistično značilna razlika med 0,1 kg in 1 kg zeolita). Ugotovili smo, da cvetni ekstrakt, pridobljen z etanolno ekstrakcijo, ne presega priporočenih dnevnih vnosov za vse tri merjene težke kovine, kar pomeni, da bi lahko na teh tleh varno pridelovali industrijsko konopljo za namene pridobivanja cvetnega ekstrakta.

Language:Slovenian
Keywords:navadna konoplja, Cannabis sativa L., potencialno toksični elementi, privzem v rastline, zeolit, klinoptilolit, cink, kadmij, svinec, Celje
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2023
PID:20.500.12556/RUL-151706 This link opens in a new window
COBISS.SI-ID:168880131 This link opens in a new window
Publication date in RUL:18.10.2023
Views:422
Downloads:36
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Secondary language

Language:English
Title:Concentration of zinc, cadmium and lead in roots, leaves, inflorescences and flower extract in hemp (Cannabis sativa), grown in Celje valley
Abstract:
Heavy metals such as zinc (Zn), cadmium (Cd) and lead (Pb) are frequently found in excessive concentrations in soils. The aim of this study was to assess the transfer of Zn, Cd and Pb by hemp grown in the Celje Basin in soils with elevated Zn, Cd and Pb concentrations. A block field experiment with seedlings was set up in the growing season of 2021, with 4 varieties of common hemp from the European Variety List (Carmagnola Selected, Elleta Campana, Finola and Tiborszallasi) and 4 doses of zeolite (0 kg, 0.1 kg, 0.5 kg and 1 kg) added to the planting hole prior to planting. At maturity of the female plants, the plants were harvested and the concentrations of Zn, Cd and Pb were measured in the roots, leaves and inflorescence, as well as in the inflorescence extract. The highest Zn concentrations were measured in leaves (55.6-254.6 mg/kg dry matter (DM)), followed by inflorescences (74.6 to 182.5 mg/kg DM) and finally roots (52.1 to 93.9 mg/kg DM). The highest concentrations of Cd were measured in the roots (0,7 to 1,4 mg/kg SS), followed by the inflorescence (0,2 to 0,5 mg/kg SS) and the lowest in the leaves (0,15 to 0,45 mg/kg SS). The highest concentrations of Pb were measured in the roots (3,4 to 6,2 mg/kg SS), followed by the leaves (0,49 to 1,99 mg/kg SS) and the lowest in the inflorescence (0,3 to 1 mg/kg SS). No statistically significant differences were observed in the effect of different zeolite doses on Zn, Cd and Pb concentrations, except for Pb concentrations in inflorescences, where the only significant difference was found between 0,1 kg and 1 kg zeolite dose. The inflorescence extract obtained by ethanol extraction does not exceed the recommended daily intakes for all three heavy metals studied, which means that hemp could be safely grown on this soil for the purpose of flower extract production.

Keywords:hemp, Cannabis sativa L., potential toxic elements, plant uptake, zeolite, clinoptilolite, zinc, cadmium, lead, Celje

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