Nastajanje lesa vinske trte (Vitis vinifera) na sušnih rastiščih na območju Neaplja v Italliji

Pivk, Meta (author), Balzano, Angela (mentor), Merela, Maks (comentor)

S pomočjo orodja Trephor smo na vinorodnih območjih v bližini Neaplja v južni Italiji, v obdobju od 23. februarja do 31. avgusta 2022, odvzeli približno 200 mikroizvrtkov iz stebel vinske trte (Vitis vinifera). Vzorčili smo na treh področjih z različno tretiranimi podlagami: naravno brez posega (NAT), sejanje drugih rastlin (trav) pod trto (SEM) in obdelano z zasipanjem trte z zemljino (LAV). Iz mikro izvrtkov smo pripravili mikroskopske preparate, ki so vsebovali floem, kambij in les. Prečne prereze tkiv vinske trte smo preučili s svetlobnim mikroskopom Zeiss Axio Imager 2 in programom za analizo sliko ZEN Pro 3.3. Spremljali smo število celic v kambiju, nastajanje lesa (postkambijalno rast, odlaganje in lignifikacijo celičnih sten in zrele celice). Pridobljene podatke smo analizirali. Upoštevajoč klimatske pogoje smo poiskali zvezo med nastajanjem lesa in kultivacijo (tretiranje tal) pri vinski trti. Kambijeva aktivnost se je na SEM in NAT pričela 3. maja 2022, na LAV pa v sredini maja. Diferenciacija celic lesa, trahej in vlaken je bila zaključena 31. avgusta 2022. Povprečna širina branik v lesu vinske trte nastalih v letu 2022 je bila 1119,24 μm (LAV), 775,79 μm (SEM) in 654,6 μm (NAT). Povprečne temperatura v letu 2022 je bila 15,12 °C, letna količina padavin pa 1191,8 mm.

• Language: Slovenian
• Keywords: Vitis vinifera, les, kambij, ksilogeneza, Neapelj, Italija
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [M. Pivk]
• Year: 2023
• PID: 20.500.12556/RUL-150424
• UDC: 630*811.13
• COBISS.SI-ID: 166571779
• Publication date in RUL: 17.09.2023

Secondary language

• Language: English
• Title: Wood formation in grapevine (Vitis vinifera) drom a drought-prone environment in the area of Naples in Italy

Abstract

Using the Trephor tool, approximately 200 micro-cores were collected from grapevine (Vitis vinifera) stems in vineyards near Naples in southern Italy between February 23 and August 31, 2022. Samples were collected in three areas with different treated rootstocks: natural without intervention (NAT), sowing of other plants under the vine (different grass species) (SEM), and treated vines with soil amendment (LAV). Microscopic slides were made from the micro-cores. They contained phloem, cambium and wood. Cross sections of the tissue were examined using a Zeiss Axio Imager 2 light microscope and the ZEN Pro 3.3 image analysis software. The number of cells in the cambium and wood formation phases (zones with cells in the stages of post-cambial growth, cell wall deposition and lignification, and maturation) were measured. The data obtained were analysed and the relationships between wood formation and cultivation (soil treatment) were assessed, taking into account climatic conditions. Cambium activity started on May 3, 2022 in SEM and NAT and in mid-May in LAV. All vessels and fibres were fully differentiated by August 31. The average width of tree rings in 2022 was 1119.24 μm (LAV), 775.79 μm (SEM), and 654.6 μm (NAT). In 2022, the average annual temperature was 15.12 °C and the total precipitation was 1191.8 mm.

• Keywords: Vitis vinifera, wood, cambium, xylogenesis, Naples, Italy