Ultra high-performance liquid chromatography is a newer version of liquid chromatography. Compared to HPLC systems, the separations have improved, which are shorter time, better resolution, and sensitivity. UHPLC technology is a column chromatography, so columns with stationary phase particles of 2 μm in diameter have been developed and flows do not exceed than 1 mL/min. The pre-column pressure reaches around 600 bar, which is a prerequisite for the mobile phase to pass through the column. I developed a method on a high-resolution liquid chromatography system. Checked the reproducibility of the solution, the repeatability of the measuring instrument and prepared the calibration curve. Then I conducted an experiment with a validated method to check the stability of kynurenic acid. Kynurenic acid is formed as a metabolic product of the amino acid L-tryptophan. The reaction is catalyzed by the enzyme kynurenine oxoglutarate transaminase. The presence of kynurenic acid has been demonstrated in various parts of living organisms, in the digestive tract, blood, peripheral nervous system and brain. Studies have shown that kynurenic acid acts antioxidant and anti-inflammatory by blocking amino acid receptors. Kynurenic acid not only affects organisms, but is also found in foods such as potatoes, broccoli, numerous spices, and herbs. An extremely high concentration of kynurenic acid is found in honey, especially in Chestnut´s. The stability of kynurenic acid is affected by the conditions to which the solution is exposed, and the medium added to the solution. The kynurenic acid solution is stable in acidic and alkaline mediums and at elevated temperatures, based on studies carried out. However, in the peroxide medium and exposure of the solution to sunlight, kynurenic acid is not stable.
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