Terapevtska uporaba avtolognih fibroblastov, gojenih in vitro, za izboljšanje videza deformacij obraznih potez: sistematični pregled in analiza globalnih trendov izvajanja kliničnih raziskav

Furman, Nina (author), Jeras, Matjaž (mentor)

Dermalni fibroblasti so celice mezenhimskega izvora in njihova ključna vloga je sinteza strukturnih elementov kože, kot so kolagen, retikularna in elastična vlakna ter glikozaminkoglikani (GAG). Pomembno vlogo imajo pri celjenju ran in obnovi kože. Razumevanje regenerativnega potenciala dermalnih fibroblastov za obnovo kože je privedlo do razvoja celičnih terapij za različne terapevtske indikacije, vključno z opeklinami, kožnimi ranami, alopecijo in kozmetičnimi posegi. Fibroblasti so ključni pri določanju regionalne identitete kože, odvisno od njihovega izvora v različnih anatomskih mestih. Tako imajo dermalni fibroblasti iz različnih mikroanatomskih predelov različne funkcionalnosti. V spletnih podatkovnih bazah Clinicaltrials.gov, Semanticsholar.org in Cochrane library smo s pomočjo vnaprej določenih iskalnih nizov poiskali klinične raziskave na področju vpliva in vitro gojenih avtolognih fibroblastov, injiciranih v kožo. Vključitveni kriterij je vseboval klinične študije, ki so raziskovale vpliv injiciranih avtolognih fibroblastov na kožo obraza z namenom odpravljanja deformacij obraznih potez, med katere uvrščamo starostne gube (nazolabialne gube, periorbitalne gube, gube na čelu in vratu ...) ter brazgotine, nastale zaradi aken. Pri pregledu smo se omejili na prosto dostopne klinične raziskave, napisane v angleškem jeziku. V sistematičnem pregledu literature smo pridobili skupno 12 terapevtsko obravnavanih kliničnih raziskav, ki so ustrezale vsem vključitvenim in izključitvenim kriterijem. Večina kliničnih raziskav, ki smo jih analizirali, je bila randomiziranih, večskupinskih in dvojno slepih. V raziskavah je sodelovalo v povprečju 105,3 udeležencev. Večina študij je potrdila učinkovitost injiciranih avtolognih fibroblastov na kožo obraza z zelo majhno pojavnostjo neželenih učinkov. Področje uporabe gojenih avtolognih fibroblastov za odpravljanje deformacij obraznih potez je razmeroma novo, vendar učinkovito in nakazuje velik potencial za terapevtsko uporabo.

• Language: Slovenian
• Keywords: avtologni fibroblasti, terapevtska uporaba, starostne gube, brazgotine po aknah
• Work type: Bachelor thesis/paper
• Organization: FFA - Faculty of Pharmacy
• Year: 2023
• PID: 20.500.12556/RUL-149612
• Publication date in RUL: 08.09.2023

FURMAN, Nina, 2023, Terapevtska uporaba avtolognih fibroblastov, gojenih in vitro, za izboljšanje videza deformacij obraznih potez: sistematični pregled in analiza globalnih trendov izvajanja kliničnih raziskav [online]. Bachelor’s thesis. [Accessed 23 March 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=149612

Secondary language

• Language: English
• Title: Therapeutic use of in vitro cultured autologous fibroblasts for the improvement of facial contour deformities: systematic review and analysis of global trends in performing clinical studies

Abstract

Dermal fibroblasts are cells of mesenchymal origin and their key role is the synthesis of structural elements of the skin, such as collagen, reticular and elastic laminae and GAGs. They play an important role in wound healing and skin regeneration. Understanding the regenerative potential of dermal fibroblasts for skin repair has led to the development of cell therapies for a variety of therapeutic indications, including burns, skin wounds, alopecia and cosmetic procedures. Fibroblasts are crucial in determining the regional identity of the skin, depending on their origin in different anatomical sites. Thus, dermal fibroblasts from different microanatomical compartments have different functionalities. Clinical studies on the impact of in vitro cultured autologous fibroblasts injected into the skin were searched in the online databases Clinicaltrials.gov, Semanticsholar.org and the Cochrane library using predefined search strings. The inclusion criteria included clinical studies investigating the effect of injected autologous fibroblasts on facial skin to correct facial deformities, including age-related wrinkles (nasolabial folds, periorbital wrinkles, forehead and neck wrinkles, etc.) and acne scars. The review was limited to freely available clinical studies written in English. In a systematic review of literature, we obtained a total of 12 clinical studies that were focused on therapeutic treatments and adhered to all inclusion and exclusion criteria. Most of the clinical trials we analyzed were randomized, multi-group and double-blind. On average, 105.3 participants took part in the trials. Most studies have confirmed the efficacy of injected autologous fibroblasts on facial skin with a very low incidence of side effects. The field of application of cultured autologous fibroblasts for the correction of facial contour deformities is relatively new, but effective and indicates great potential for therapeutic use.

• Keywords: autologous fibroblasts, therapeutic use, wrinkles, acne scars