Spektroskopske raziskave kemijske denaturacije lizocima

Martinčič, Laura (author), Šarac, Bojan (mentor)

Namen diplomske naloge je bil preučiti, kako dodatek gvanidin hidroklorida (GdHCl) vpliva na kemijsko denaturacijo proteina lizocima pri treh različnih temperaturah. Sam potek kemijske denaturacije lizocima sem spremljala z merjenjem fluorescence. Na podlagi meritev sem izračunala stopnjo denaturacije lizocima in standardno prosto Gibbsovo energijo ter standardno entalpijo za denaturacijo lizocima. Vrednosti sem primerjala z vrednostmi, pridobljenih iz toplotne denaturacije ter literature.

• Language: Slovenian
• Keywords: proteini, lizocim, gvanidin hidroklorid, fluorescenca, denaturacija
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2023
• PID: 20.500.12556/RUL-149559
• COBISS.SI-ID: 164550147
• Publication date in RUL: 07.09.2023

Secondary language

• Language: English
• Title: Spectroscopic research of chemical denaturation of lysozyme

Abstract

The aim of the thesis was to examine how the addition of guanidine hydrochloride (GdHCl) affects the chemical denaturation of lysozyme protein at three different temperatures. The progress of lysozyme's chemical denaturation was monitored by measuring fluorescence. Based on the measurements, I calculated the degree of lysozyme denaturation and the standard Gibbs energy and standard enthalpy for denaturation of lysozyme. The values were compared to the values obtained from thermal denaturation and literature.

• Keywords: proteins, lysozyme, guanidine hydrochloride, fluorescence, denaturation