In biological control, living organisms are used for pest management. The aim of this master's thesis is to determine how antagonistic fungi from the genus Trichoderma, isolated from the biostimulants Pannon Starter® Perfect Pro and Trifender® pro WP, affect the growth of mycelium and the formation of sclerotia of pathogenic fungi - S. sclerotiorum, S. minor, S. cepivorum, A. rolfsii, M. phaseolina - and what are the possibilities for further use of biological control agents in agriculture. The hyperparasitic fungus C. minitans, isolated from Contans® WG, was used as a positive control. We performed an experiment of dual culture, where one of the pathogenic fungi was inoculated on the culture medium at different times (day 0, day 3 or day 7) in combination with one of the antagonistic fungi. The experiment was conducted at two temperatures, 15 and 25 °C. For 10 days we marked the daily growth of mycelium of fungi. We found that the temperature had a great influence on the growth of the mycelium and also on the interaction of the two fungi Inoculation of the pathogenic fungus on the culture medium to the antagonistic fungus on day 3 or day 7 did not show any visible difference from 0. day inoculation of fungi on medium. Mycoparasitism of sclerotia of pathogenic fungi by the Trichoderma spp. was researched in two different ways. First by adding a suspension of biostimulants to the sclerotia of the pathogen, which proved to be more reliable, and second, by subsequent visual evaluation of sclerotia formed in dual culture. In addition, we included a pot experiment, where sclerotia of S. sclerotiorum and S. minor and suspensions of previously used biostimulants were added to lettuce seedlings. Our research is a good basis for further experiments to search new biotic protection products using fungi of the Trichoderma genus.
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