Oxidative stress markers cannot be used as endometriosis biomarkers in infertile patients

Janša, Vid (author), Osredkar, Joško (author), Verdenik, Ivan (author), Lanišnik-Rižner, Tea (author), Ban, Helena (author)

Objectives: Endometriosis is a common benign gynaecological disease that significantly compromises the quality of life of patients. To date, invasive surgery is the method of choice to visually and histologically confirm endometriosis. Thus, there is a major interest to develop noninvasive diagnostic tools. Oxidative stress is one of the proposed mechanisms of pathogenesis and may be involved in pelvic pain, dysmenorrhea, dyspareunia, and infertility in endometriosis patients. Thus, markers of oxidative stress may serve as diagnostic biomarkers for endometriosis. Design: This prospective case–control study assessed erythrocyte superoxide dismutase (SOD), erythrocyte glutathione peroxidase (GPX), serum hexanoyl lysine (HEL) and peritoneal fluid HEL. Participants/Materials, Setting, and Methods: We enrolled 86 women with primary infertility; the case group included 57 women with endometriosis, and the control group included 29 women with unexplained primary infertility. All the patients underwent laparoscopy, and the diagnosis was confirmed histologically. RANDOX and RANSEL reagents were used to determine the levels of SOD and GPX, respectively, and ELISA was used to determine the levels of HEL. Results: We found no statistically significant differences in the erythrocyte levels of GPX (p value 0.623) or SOD (p value 0.122) or the serum or peritoneal fluid levels of HEL (p value 0.562 and 0.329 accordingly). Conclusions: SOD, GPX, and HEL levels most likely do not differ between patients with unexplained infertility and patients with endometriosis.

• Language: English
• Keywords: oxidative stress biomarkers, endometriosis, superoxide dismutase, glutathione peroxidase, hexanoyl lysine
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine; FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 6 str.
• Numbering: Vol. 39, no. 1
• PID: 20.500.12556/RUL-148655
• UDC: 612.015:618.14
• ISSN on article: 0951-3590
• DOI: 10.1080/09513590.2023.2242956
• COBISS.SI-ID: 162356739
• Publication date in RUL: 29.08.2023

Record is a part of a journal

• Title: Gynecological endocrinology
• Shortened title: Gynecol. endocrinol.
• Publisher: Taylor and Francis Group
• ISSN: 0951-3590
• COBISS.SI-ID: 9017561

Licences

• License: CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
• Link: http://creativecommons.org/licenses/by-nc/4.0/
• Description: A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.

Secondary language

• Language: Slovenian
• Keywords: biomarkerji oksidativnega stresa, superoksid dismutaza, glutation peroksidaza, heksanoil lizin, oksidativni stres, porodništvo, endometrioza

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0390
• Name: Funkcijska genomika in biotehnologija za zdravje