Proteomics of the haemolymph of the terrestrial crustacean Porcellio scaber reveals components of its innate immunity under baseline conditions

Jemec Kokalj, Anita (author), Leonardi, Adrijana (author), Perc, Valentina (author), Dolar, Andraž (author), Drobne, Damjana (author), Križaj, Igor (author)

The terrestrial crustacean Porcellio scaber is an established test organism in environmental research. We analysed the haemolymph proteome of P. scaber using a classical proteomic approach based on one-dimensional gel electrophoresis and tandem mass spectrometry. Using a publicly available protein database and our P. scaber transcriptome data, we have identified 76 proteins involved in cytoskeleton formation, protein degradation, vesicular transport, genetic information processing, detoxification, carbohydrate and lipid metabolism reflecting haemocyte metabolic activity, active intracellular transport, and intercellular communication. Compared with the data reported for other crustaceans, 28 of these P. scaber proteins have been linked to its immunity, among them hemocyanin, α-2-macroglobulin, phenoloxidase 3, superoxide dismutase, glutathione S-transferase, haemolymph clottable protein, and histones H4 and H2B. Our results thus provide a firm base for studying the innate immune response of P. scaber at the level of the haemolymph proteome. This knowledge is of particular importance in ecotoxicity studies with various environmental stressors where understanding physiological changes is important to reveal possible modes of action.

• Language: English
• Keywords: woodlice, Porcellio scaber, haemolymph, proteome, immunity, mass spectrometry
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: Str. 12-21
• Numbering: Vol. 213
• PID: 20.500.12556/RUL-148279
• UDC: 577
• ISSN on article: 0300-9084
• DOI: 10.1016/j.biochi.2023.05.007
• COBISS.SI-ID: 152020483
• Publication date in RUL: 09.08.2023

Record is a part of a journal

• Title: Biochimie
• Shortened title: Biochimie
• Publisher: Elsevier
• ISSN: 0300-9084
• COBISS.SI-ID: 4254474

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• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
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